Improvement of sporulation in the yeast Yarrowia lipolytica.

Antonie van Leeuwenhoek ·Vol. 51 ·No. 2 ·1985-00-00 ·Pages 167-77

Barth G, Weber H

Abstract

Strains of Yarrowia lipolytica forming exclusively spherical ascospores were developed through inbreeding. These strains are more suitable for micromanipulation than other inbred strains forming helm-shaped ascospores. External factors affecting sporulation frequency and tetrad formation in this yeast were investigated. Optimal formation of complete tetrads occurred at a narrow range of pH values around 6.0. Citrate was found to stimulate sporulation strongly. A synthetic medium containing citrate was developed to obtain standard conditions for maximum sporulation.

MeSH Terms

Ascomycota/genetics,physiology Citrates/metabolism Citric Acid Conjugation, Genetic Crosses, Genetic Culture Media Hydrogen-Ion Concentration Microscopy, Electron Phenotype Spores, Fungal/genetics,physiology,ultrastructure

Chemicals

Citrates Culture Media Citric Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Barth G

Weber H

References (16)

16 references, click to expand

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Article Info

Journal

Antonie van Leeuwenhoek

Abbr.

Antonie Van Leeuwenhoek

ISSN

0003-6072

DOI

10.1007/BF02310010

Published

1985-00-00

Pages

167-77

Language

English

Region

Netherlands

NLM ID

0372625

Subset

External Links

PubMed source DOI: 10.1007/BF02310010 View journal details

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Improvement of sporulation in the yeast Yarrowia lipolytica.

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