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PMID: 2156435 Published · ppublish English Journal Article

The detection of Epstein-Barr virus in hairy cell leukemia cells by in situ hybridization.

The American journal of pathology ·Vol. 136 ·No. 3 ·1990-03-00 ·Pages 717-23

Wolf BC, Martin AW, Neiman RS, Janckila AJ, Yam LT, Caracansi A, Leav BA, Winpenny R, Schultz DS, Wolfe HJ

Abstract

Epstein-Barr virus (EBV) has been implicated in the pathogenesis of several B-cell lymphoid proliferations. Because patients with hairy cell leukemia (HCL) have a high incidence of seropositivity for EBV antigens, we studied the cells of HCL for evidence of EBV infection using in situ hybridization techniques. EBV mRNA was detected in the tumor cells in four of six cases using a radiolabeled RNA probe. Confirmatory serologic data were available in three cases in which the viral DNA was detected and in one negative case. Our results suggest that EBV infection may have a pathogenetic role in this disorder.

MeSH Terms
Adult Antigens, Viral/immunology DNA, Viral/genetics Herpesvirus 4, Human/genetics,immunology,isolation & purification Humans Leukemia, Hairy Cell/immunology,microbiology,pathology Male Microscopy, Electron Middle Aged Nucleic Acid Hybridization RNA, Messenger/genetics RNA, Viral/genetics Spleen/microbiology,pathology,ultrastructure
Chemicals
Antigens, Viral DNA, Viral RNA, Messenger RNA, Viral
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wolf B C
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts.
Martin A W
Neiman R S
Janckila A J
Yam L T
Caracansi A
Leav B A
Winpenny R
Schultz D S
Wolfe H J
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1990-03-00
Pages
717-23
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1877477
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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