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PMID: 2136735 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of the C operon transcript of bacteriophage Mu.

Journal of bacteriology ·Vol. 172 ·No. 1 ·1990-01-00 ·Pages 361-71

Stoddard SF, Howe MM

Abstract

Mu transcription occurs in three phases: early, middle, and late. Middle transcription occurs in the region of the C gene, which encodes the transactivator for late transcription. A middle promoter, Pm, was previously localized between 0.28 and 1.2 kilobase pairs upstream of C. We used S1 nuclease mapping with both unlabeled and radiolabeled capped RNAs from induced lysogens to characterize C transcription and identify its promoter. The C transcription initiation site was localized to a 4-base-pair region, approximately 740 base pairs upstream of C within the region containing Pm. Transcription of C was activated between 4 and 8 min after induction of cts and Cam lysogens and increased throughout the lytic cycle. Significant C transcription did not occur in replication-defective Aam lysogens. These kinetic and regulatory characteristics identify the C transcript as a middle RNA species and demonstrate that Pm is the C promoter. DNA sequence analysis of the Pm region showed a good -10, but poor -35, site homology to the Escherichia coli RNA polymerase consensus sequence. In addition, the sequence demonstrated that C is the distal gene in a middle operon containing several open reading frames. S1 mapping also showed an upstream transcript with a 3' end in the Pm region at a sequence strongly resembling a Rho-independent terminator. The regulatory characteristics of this RNA are consistent with this terminator, t9.2, being the early operon terminator.

MeSH Terms
Bacteriophage mu/genetics Base Sequence Chromosome Mapping DNA Replication Molecular Sequence Data Operon RNA, Viral/analysis Transcription, Genetic
Chemicals
RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stoddard S F
Department of Microbiology and Immunology, University of Tennessee-Memphis 38163.
Howe M M
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45 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.1.361-371.1990
Published
1990-01-00
Pages
361-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208440
Subset
IM
Grants
NIAID NIH HHS · AI12731 · United States
NIAID NIH HHS · AI24774 · United States
Databases
GENBANK
M13657, X02992, Y00419
External Links
PubMed source DOI: 10.1128/jb.172.1.361-371.1990 PMC Full Text View journal details
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