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PMID: 2124971 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protein secretion in gram-negative bacteria: transport across the outer membrane involves common mechanisms in different bacteria.

The EMBO journal ·Vol. 9 ·No. 13 ·1990-12-00 ·Pages 4323-9

Filloux A, Bally M, Ball G, Akrim M, Tommassen J, Lazdunski A

Abstract

The xcp genes are required for protein secretion by Pseudomonas aeruginosa. They are involved in the second step of the process, i.e. the translocation across the outer membrane, after the exoproteins have reached the periplasm in a signal peptide dependent fashion. The nucleotide sequence of a 2.5 kb DNA fragment containing xcp genes showed at least two complete open reading frames, potentially encoding proteins with molecular weights of 41 and 19 kd. Products with these apparent molecular weights were identified after expression of the DNA fragment in vitro and in vivo. Subcloning and complementation experiments showed that both proteins are required for secretion. The two products are located in the inner membrane and share highly significant homologies with the PulL and PulM proteins which are required for the specific secretion of pullulanase in Klebsiella pneumoniae. These homologies reveal the existence of a common mechanism for protein secretion in Pseudomonas aeruginosa and Klebsiella pneumoniae.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics,metabolism Bacterial Proteins/genetics Base Composition Base Sequence Biological Transport Cell Membrane/metabolism DNA, Bacterial/chemistry Klebsiella pneumoniae/genetics,metabolism Membrane Proteins Membrane Transport Proteins Molecular Sequence Data Pseudomonas aeruginosa/genetics,metabolism Restriction Mapping
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA, Bacterial Membrane Proteins Membrane Transport Proteins xcpY protein, Pseudomonas aeruginosa xcpZ protein, Pseudomonas aeruginosa
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Filloux A
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
Bally M
Ball G
Akrim M
Tommassen J
Lazdunski A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
4323-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552217
Subset
IM
Databases
GENBANK
X56183
External Links
PubMed source PMC Full Text View journal details
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