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PMID: 2118109 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of apoptosis in interleukin-3-dependent hemopoietic cells by interleukin-3 and calcium ionophores.

The EMBO journal ·Vol. 9 ·No. 9 ·1990-09-00 ·Pages 2997-3002

Rodriguez-Tarduchy G, Collins M, López-Rivas A

Abstract

An immortalized interleukin-3 (IL-3)-dependent progenitor cell line, BAF-3, undergoes programmed cell death (apoptosis) when deprived of IL-3. This program is characterized by an early degradation of DNA into oligonucleosome-length fragments that precedes by several hours the loss of cell viability. In the absence of IL-3, DNA fragmentation and cell death can be prevented by the calcium ionophores A23187 (1 microM) and ionomycin (0.5 microM). This addition of calcium ionophore maintains cell viability while reversibly arresting the cell cycle. Apoptosis by growth factor deprivation is also a mechanism of cell elimination in bone marrow cells removed from the stromal micro-environment, as DNA fragmentation and cell death was shown to take place in primary cultures of IL-3-responsive bone marrow cells after IL-3 removal.

MeSH Terms
Animals Bone Marrow Cells Calcimycin/pharmacology Calcium/physiology Cell Cycle/drug effects Cell Survival/drug effects Cells, Cultured DNA/isolation & purification DNA Replication/drug effects Hematopoietic Stem Cells/cytology,drug effects,immunology Interleukin-3/pharmacology Ionomycin/pharmacology Mice
Chemicals
Interleukin-3 Calcimycin Ionomycin DNA Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rodriguez-Tarduchy G
Instituto de Investigaciones Biomédicas CSIC, Facultad de Medicina UAM, Madrid, Spain.
Collins M
López-Rivas A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-09-00
Pages
2997-3002
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552017
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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