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PMID: 3502088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interleukin 3-specific tyrosine phosphorylation of a membrane glycoprotein of Mr 150,000 in multi-factor-dependent myeloid cell lines.

The EMBO journal ·Vol. 6 ·No. 13 ·1987-12-20 ·Pages 3979-84

Koyasu S, Tojo A, Miyajima A, Akiyama T, Kasuga M, Urabe A, Schreurs J, Arai K, Takaku F, Yahara I

Abstract

Tyrosine phosphorylation of cellular proteins induced by various hematopoietic growth factors such as interleukin 3 (IL3), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL4) was studied in several multi-factor-dependent myeloid cell lines. Among the growth factors, IL3 specifically induced rapid tyrosine phosphorylation of a membrane glycoprotein of mol. wt 150 kd (gpp150) in the IL3-dependent cell lines, IC2 and DA-1. The IL3-induced tyrosine phosphorylation of gpp150 was detected within 30 s, reached a maximum at 3 min and decreased thereafter. The concentration of IL3 required for half-maximum stimulation of gpp150 tyrosine phosphorylation with 2.5 x 10(6)/ml cells was approximately 200 pM, which is the same as the dissociation constant for 125I-labeled IL3 binding. gpp150 was constitutively phosphorylated on tyrosine residue(s) in growth factor independent variants, IC2Tr and DA-1Tr, derived from IC2 and DA-1 respectively. Neither variant synthesized IL3. The present findings suggest that tyrosine phosphorylation of gpp150 is a critical event involved in both IL3-dependent and -independent growth.

MeSH Terms
Animals Cell Line DNA Replication/drug effects Female Growth Substances/pharmacology Interleukin-3/pharmacology Membrane Glycoproteins/metabolism Mice Mice, Inbred DBA Molecular Weight Phosphorylation Recombinant Proteins/pharmacology Tyrosine
Chemicals
Growth Substances Interleukin-3 Membrane Glycoproteins Recombinant Proteins Tyrosine
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Koyasu S
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
Tojo A
Miyajima A
Akiyama T
Kasuga M
Urabe A
Schreurs J
Arai K
Takaku F
Yahara I
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-12-20
Pages
3979-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553877
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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