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PMID: 2531081 Published · ppublish English Journal Article

EGF induces differentiation of an IL-3-dependent cell line expressing the EGF receptor.

The EMBO journal ·Vol. 8 ·No. 12 ·1989-12-01 ·Pages 3677-84

Wang HM, Collins M, Arai K, Miyajima A

Abstract

Interleukin 3 (IL-3) is a T cell-derived lymphokine that supports the growth and development of hematopoietic cells. Tyrosine phosphorylation has been suggested to play an important role in IL-3-dependent cell proliferation. To test whether a growth factor receptor carrying a tyrosine kinase can be functional in IL-3 dependent cells, we used a retroviral vector to introduce the human EGF receptor into a murine IL-3-dependent pre-mast cell line, IC2. The EGF receptors expressed on the infected clones bind EGF with both high and low affinities. EGF stimulates the infected cells for a short term growth response. In the presence of IL-3 and EGF, infected clones differentiate into more mature mast cells characterized by increases in intracellular granulation and histamine content. This differentiation is reversible when EGF is removed. EGF induces tyrosine phosphorylation of several cellular proteins and the expression of oncogenes c-fos and c-myc, in a manner analogous to IL-3 stimulation. These results indicate that the EGF receptor is functional in the pre-mast IC2 cells; EGF can support short-term proliferation and activates the signals that induce cell differentiation. Thus, EGF receptor-expressing IC2 cells provide a unique cellular system for in vitro study of mast cell differentiation.

MeSH Terms
Animals Calcimycin/pharmacology Cell Differentiation/drug effects Cell Division/drug effects Cell Line Epidermal Growth Factor/pharmacology ErbB Receptors/drug effects,metabolism Humans Interleukin-3/pharmacology Mast Cells/drug effects,metabolism,physiology Mice Oncogene Proteins/biosynthesis Phosphorylation Tetradecanoylphorbol Acetate/pharmacology Tyrosine/metabolism
Chemicals
Interleukin-3 Oncogene Proteins Calcimycin Tyrosine Epidermal Growth Factor ErbB Receptors Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wang H M
Department of Molecular Biology, DNAX Research Institute, Palo Alto, CA 94304.
Collins M
Arai K
Miyajima A
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-12-01
Pages
3677-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC402050
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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