Home LiteratureArticle Details
PMID: 2115670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a human cDNA encoding a widely expressed and highly conserved cysteine-rich protein with an unusual zinc-finger motif.

Nucleic acids research ·Vol. 18 ·No. 13 ·1990-07-11 ·Pages 3871-9

Liebhaber SA, Emery JG, Urbanek M, Wang XK, Cooke NE

Abstract

A human term placental cDNA library was screened at low stringency with a human prolactin cDNA probe. One of the cDNAs isolated hybridizes to a 1.8 kb mRNA present in all four tissues of the placenta as well as to every nucleated tissue and cell line tested. The sequence of the full-length cDNA was determined. An extended open reading frame predicted an encoded protein product of 20.5 kDa. This was directly confirmed by the in vitro translation of a synthetic mRNA transcript. Based upon the characteristic placement of cysteine (C) and histidine (H) residues in the predicted protein structure, this molecule contains four putative zinc fingers. The first and third fingers are of the C4 class while the second and fourth are of the C2HC class. Based upon sequence similarities between the first two and last two zinc fingers and sequence similarities to a related rodent protein, cysteine-rich intestinal protein (CRIP), these four finger domains appear to have evolved by duplication of a preexisting two finger unit. Southern blot analyses indicate that this human cysteine-rich protein (hCRP) gene has been highly conserved over the span of evolution from yeast to man. The characteristics of this protein suggest that it serves a fundamental role in cellular function.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Blotting, Southern Cysteine/analysis DNA/genetics DNA-Binding Proteins/genetics Female Humans Metalloproteins/genetics Molecular Sequence Data Placenta Placental Hormones/genetics Pregnancy Proteins/biosynthesis,genetics,isolation & purification Prolactin/genetics RNA, Messenger/analysis Sequence Homology, Nucleic Acid Zinc
Chemicals
DNA-Binding Proteins Metalloproteins Placental Hormones Pregnancy Proteins RNA, Messenger Prolactin DNA Zinc Cysteine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liebhaber S A
Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia 19104.
Emery J G
Urbanek M
Wang X K
Cooke N E
References (51)
51 references, click to expand
  1. DNA binding specificity of steroid receptors.
    Cell. 1989 Jun 30;57(7):1065-8 PMID: 2661016
  2. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  3. Poly(ADP-ribose)polymerase: a novel finger protein.
    Nucleic Acids Res. 1989 Jun 26;17(12):4689-98 PMID: 2501758
  4. DNA analysis in the diagnosis of hemoglobin disorders.
    Methods Enzymol. 1981;76:805-17 PMID: 6276651
  5. Characterization of the two forms of rat placental lactogen (rPL): rPL-I and rPL-II.
    Endocrinology. 1982 Dec;111(6):1862-6 PMID: 7140637
  6. Expression of the prolactin gene in human decidua-chorion.
    Endocrinology. 1983 Mar;112(3):1133-4 PMID: 6185329
  7. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  8. Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.
    J Biol Chem. 1983 Dec 10;258(23):14120-5 PMID: 6196359
  9. Targeting of E. coli beta-galactosidase to the nucleus in yeast.
    Cell. 1984 Apr;36(4):1057-65 PMID: 6323016
  10. Molecular cloning and nucleotide sequence of DNA complementary to human decidual prolactin mRNA.
    J Biochem. 1984 May;95(5):1491-9 PMID: 6146607
  11. A short amino acid sequence able to specify nuclear location.
    Cell. 1984 Dec;39(3 Pt 2):499-509 PMID: 6096007
  12. Translationally associated helix-destabilizing activity in rabbit reticulocyte lysate.
    J Biol Chem. 1984 Dec 25;259(24):15597-602 PMID: 6096379
  13. The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
    FEBS Lett. 1985 Jul 8;186(2):271-4 PMID: 4007166
  14. Identification of proliferin mRNA and protein in mouse placenta.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4356-9 PMID: 3859868
  15. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  16. Amino acid sequence homology in gag region of reverse transcribing elements and the coat protein gene of cauliflower mosaic virus.
    Nucleic Acids Res. 1986 Jan 24;14(2):623-33 PMID: 2418414
  17. Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2516-20 PMID: 3085096
  18. Nucleotide sequence of the ARGRII regulatory gene and amino acid sequence homologies between ARGRII PPRI and GAL4 regulatory proteins.
    Eur J Biochem. 1986 May 15;157(1):77-81 PMID: 3709534
  19. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.
    Science. 1980 Jul 25;209(4455):497-9 PMID: 6248960
  20. Human prolactin. cDNA structural analysis and evolutionary comparisons.
    J Biol Chem. 1981 Apr 25;256(8):4007-16 PMID: 6260780
  21. Differentiation of the mRNA transcripts originating from the alpha 1- and alpha 2-globin loci in normals and alpha-thalassemics.
    J Clin Invest. 1981 Aug;68(2):439-46 PMID: 6894931
  22. Structural features of prolactins and growth hormones that can be related to their biological properties.
    Endocr Rev. 1986 May;7(2):169-203 PMID: 3013605
  23. Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes.
    Mol Cell Biol. 1985 Oct;5(10):2720-32 PMID: 3837182
  24. Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5597-601 PMID: 3461452
  25. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  26. Gene 32 protein, the single-stranded DNA binding protein from bacteriophage T4, is a zinc metalloprotein.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8452-6 PMID: 3490667
  27. Bifunctional messenger RNAs in eukaryotes.
    Cell. 1986 Nov 21;47(4):481-3 PMID: 3779834
  28. Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.
    Nature. 1986 Nov 13-19;324(6093):163-6 PMID: 3785382
  29. Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3316-9 PMID: 3554236
  30. Molecular cloning of a prolactin-related mRNA expressed in bovine placenta.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5650-4 PMID: 3475696
  31. The sex-determining region of the human Y chromosome encodes a finger protein.
    Cell. 1987 Dec 24;51(6):1091-104 PMID: 3690661
  32. Zinc fingers: gilt by association.
    Cell. 1988 Jan 15;52(1):1-3 PMID: 3125980
  33. A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeable.
    Cell. 1988 Apr 8;53(1):25-35 PMID: 3280141
  34. The steroid and thyroid hormone receptor superfamily.
    Science. 1988 May 13;240(4854):889-95 PMID: 3283939
  35. Fingering too many proteins.
    Cell. 1988 Jun 3;53(5):675 PMID: 3370671
  36. Characterization and histologic localization of human growth hormone-variant gene expression in the placenta.
    J Clin Invest. 1989 Jun;83(6):1985-91 PMID: 2723069
  37. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  38. Helix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins.
    Trends Biochem Sci. 1989 Apr;14(4):137-40 PMID: 2499084
  39. Identification of a zinc finger protein that binds to the sterol regulatory element.
    Science. 1989 Aug 11;245(4918):640-3 PMID: 2562787
  40. The erythroid-specific transcription factor Eryf1: a new finger protein.
    Cell. 1989 Sep 8;58(5):877-85 PMID: 2776214
  41. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  42. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  43. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  44. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  45. Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.
    Cell. 1977 Feb;10(2):265-73 PMID: 837449
  46. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  47. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  48. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  49. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  50. Molecular analysis of cloned human 18S ribosomal DNA segments.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5367-71 PMID: 364477
  51. ZFX has a gene structure similar to ZFY, the putative human sex determinant, and escapes X inactivation.
    Cell. 1989 Jun 30;57(7):1247-58 PMID: 2500252
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.13.3871
Published
1990-07-11
Pages
3871-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331088
Subset
IM
Grants
NICHD NIH HHS · R01-HD25147 · United States
NCRR NIH HHS · RR01865-05 · United States
Databases
GENBANK
M33146
External Links
PubMed source DOI: 10.1093/nar/18.13.3871 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2