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PMID: 3461452 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5597-601

Henthorn PS, Knoll BJ, Raducha M, Rothblum KN, Slaughter C, Weiss M, Lafferty MA, Fischer T, Harris H

Abstract

Amino-terminal amino acid sequences (42 residues) were determined for the products of the three common alleles at the human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] gene locus. The sequences differ at position 3, which is proline in types 1 and 2 but is leucine in type 3. cDNA libraries were constructed in phage lambda gt11 and used to isolate clones covering the coding regions of types 1 and 3 cDNAs. Comparison of the deduced amino acid sequences of the types 1 and 3 proteins showed 7 differences out of 513 amino acids, each due to a single base substitution. cDNA sequence comparisons showed three silent substitutions in the coding regions and three base differences in the greater than 1 kilobase pairs of 3' untranslated sequences.

MeSH Terms
Alkaline Phosphatase/genetics Alleles Amino Acid Sequence Cloning, Molecular DNA/genetics Humans Placenta/enzymology Sequence Homology, Nucleic Acid
Chemicals
DNA Alkaline Phosphatase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Henthorn P S
Knoll B J
Raducha M
Rothblum K N
Slaughter C
Weiss M
Lafferty M A
Fischer T
Harris H
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5597
Published
1986-08-00
Pages
5597-601
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386335
Subset
IM
Grants
NIGMS NIH HHS · GM 07170 · United States
NIGMS NIH HHS · GM27018 · United States
NICHD NIH HHS · HD 07107 · United States
Databases
GENBANK
M14169, M14170
External Links
PubMed source DOI: 10.1073/pnas.83.15.5597 PMC Full Text View journal details
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