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PMID: 20976003 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Selective release of microRNA species from normal and malignant mammary epithelial cells.

PloS one ·Vol. 5 ·No. 10 ·2010-10-20 ·Pages e13515

Pigati L, Yaddanapudi SC, Iyengar R, Kim DJ, Hearn SA, Danforth D, Hastings ML, Duelli DM

Abstract

MicroRNAs (miRNAs) in body fluids are candidate diagnostics for a variety of conditions and diseases, including breast cancer. One premise for using extracellular miRNAs to diagnose disease is the notion that the abundance of the miRNAs in body fluids reflects their abundance in the abnormal cells causing the disease. As a result, the search for such diagnostics in body fluids has focused on miRNAs that are abundant in the cells of origin. Here we report that released miRNAs do not necessarily reflect the abundance of miRNA in the cell of origin. We find that release of miRNAs from cells into blood, milk and ductal fluids is selective and that the selection of released miRNAs may correlate with malignancy. In particular, the bulk of miR-451 and miR-1246 produced by malignant mammary epithelial cells was released, but the majority of these miRNAs produced by non-malignant mammary epithelial cells was retained. Our findings suggest the existence of a cellular selection mechanism for miRNA release and indicate that the extracellular and cellular miRNA profiles differ. This selective release of miRNAs is an important consideration for the identification of circulating miRNAs as biomarkers of disease.

MeSH Terms
Breast Neoplasms/metabolism,pathology Cell Line, Tumor Female Humans Mammary Glands, Human/metabolism MicroRNAs/metabolism
Chemicals
MicroRNAs
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Pigati Lucy
Department of Pathology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois, United States of America.
Yaddanapudi Sree C S
Iyengar Ravi
Kim Dong-Ja
Hearn Steven A
Danforth David
Hastings Michelle L
Duelli Dominik M
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0013515
Published
2010-10-20
Epub
2010-00-20
Pages
e13515
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2958125
Subset
IM
Grants
NCRR NIH HHS · S10 RR019325 · United States
PHS HHS · C76 HF03610-01-00 · United States
NCRR NIH HHS · S10 RR19325 · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0013515 PMC Full Text View journal details
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