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PMID: 17599088 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer-TRBP complex.

EMBO reports ·Vol. 8 ·No. 8 ·2007-08-00 ·Pages 763-9

Kawahara Y, Zinshteyn B, Chendrimada TP, Shiekhattar R, Nishikura K

Abstract

MicroRNAs (miRNAs) mediate translational repression or degradation of their target messenger RNAs by RNA interference (RNAi). The primary transcripts of miRNA genes (pri-miRNAs) are sequentially processed by the nuclear Drosha-DGCR8 complex to approximately 60-70 nucleotide (nt) intermediates (pre-miRNAs) and then by the cytoplasmic Dicer-TRBP complex to approximately 20-22 nt mature miRNAs. Certain pri-miRNAs are subject to RNA editing that converts adenosine to inosine (A --> I RNA editing); however, the fate of edited pri-miRNAs is mostly unknown. Here, we provide evidence that RNA editing of pri-miR-151 results in complete blockage of its cleavage by Dicer and accumulation of edited pre-miR-151 RNAs. Our results indicate that A --> I conversion at two specific positions of the pre-miRNA foldback structure can affect its interaction with the Dicer-TRBP complex, showing a new regulatory role of A --> I RNA editing in miRNA biogenesis.

MeSH Terms
Adenosine Deaminase/genetics Animals Base Sequence Brain/metabolism Humans Mice Mice, Mutant Strains MicroRNAs/chemistry,genetics,metabolism Molecular Sequence Data Nucleic Acid Conformation RNA Editing RNA Precursors/chemistry,metabolism RNA-Binding Proteins Ribonuclease III/chemistry,metabolism
Chemicals
MIRN151 microRNA, mouse MicroRNAs RNA Precursors RNA-Binding Proteins Ribonuclease III ADARB1 protein, human Adenosine Deaminase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kawahara Yukio
The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA. ykawahara@wistar.org
Zinshteyn Boris
Chendrimada Thimmaiah P
Shiekhattar Ramin
Nishikura Kazuko
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7401011
Email
ykawahara@wistar.org
Published
2007-08-00
Epub
2007-00-22
Pages
763-9
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1978079
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.embor.7401011 PMC Full Text View journal details
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