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PMID: 14691248 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of many microRNAs that copurify with polyribosomes in mammalian neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 1 ·2004-01-06 ·Pages 360-5

Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, Church GM, Ruvkun G

Abstract

Localized translation in mammalian dendrites may play a role in synaptic plasticity and contribute to the molecular basis for learning and memory. The regulatory mechanisms that control localized translation in neurons are not well understood. We propose a role for microRNAs (miRNAs), a class of noncoding RNAs, as mediators of neuronal translational regulation. We have identified 86 miRNAs expressed in mammalian neurons, of which 40 have not previously been reported. A subset of these miRNAs exhibits temporally regulated expression in cortical cultures. Moreover, all of the miRNAs that were tested cofractionate with polyribosomes, the sites of active translation. These findings indicate that a large, diverse population of miRNAs may function to regulate translation in mammalian neurons.

MeSH Terms
Animals Base Sequence Cell Fractionation Cells, Cultured Cerebral Cortex/cytology,metabolism Mice MicroRNAs/genetics,isolation & purification,metabolism Neuronal Plasticity Neurons/metabolism Polyribosomes/metabolism Protein Biosynthesis Rats Sequence Homology, Nucleic Acid
Chemicals
MicroRNAs
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kim John
Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Krichevsky Anna
Grad Yonatan
Hayes Gabriel D
Kosik Kenneth S
Church George M
Ruvkun Gary
References (68)
68 references, click to expand
  1. bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila.
    Cell. 2003 Apr 4;113(1):25-36 PMID: 12679032
  2. The microRNAs of Caenorhabditis elegans.
    Genes Dev. 2003 Apr 15;17(8):991-1008 PMID: 12672692
  3. The Caenorhabditis elegans hunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs.
    Dev Cell. 2003 May;4(5):625-37 PMID: 12737799
  4. The C elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable microRNA target.
    Dev Cell. 2003 May;4(5):639-50 PMID: 12737800
  5. MicroRNAs and other tiny endogenous RNAs in C. elegans.
    Curr Biol. 2003 May 13;13(10):807-18 PMID: 12747828
  6. Computational and experimental identification of C. elegans microRNAs.
    Mol Cell. 2003 May;11(5):1253-63 PMID: 12769849
  7. Embryonic stem cell-specific MicroRNAs.
    Dev Cell. 2003 Aug;5(2):351-8 PMID: 12919684
  8. The establishment of polarity by hippocampal neurons in culture.
    J Neurosci. 1988 Apr;8(4):1454-68 PMID: 3282038
  9. The distribution of synapsin I and synaptophysin in hippocampal neurons developing in culture.
    J Neurosci. 1991 Jun;11(6):1617-26 PMID: 1904480
  10. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.
    Cell. 1993 Dec 3;75(5):843-54 PMID: 8252621
  11. Cell fate determination in the vertebrate retina.
    Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):589-95 PMID: 8570600
  12. A requirement for local protein synthesis in neurotrophin-induced hippocampal synaptic plasticity.
    Science. 1996 Sep 6;273(5280):1402-6 PMID: 8703078
  13. The embryonic central nervous system lineages of Drosophila melanogaster. I. Neuroblast lineages derived from the ventral half of the neuroectoderm.
    Dev Biol. 1996 Oct 10;179(1):41-64 PMID: 8873753
  14. The 3'-untranslated region of CaMKII alpha is a cis-acting signal for the localization and translation of mRNA in dendrites.
    Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13250-5 PMID: 8917577
  15. Translocation of RNA granules in living neurons.
    J Neurosci. 1996 Dec 15;16(24):7812-20 PMID: 8987809
  16. The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA.
    Cell. 1997 Mar 7;88(5):637-46 PMID: 9054503
  17. The embryonic central nervous system lineages of Drosophila melanogaster. II. Neuroblast lineages derived from the dorsal part of the neuroectoderm.
    Dev Biol. 1997 Sep 15;189(2):186-204 PMID: 9299113
  18. Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.
    J Mol Biol. 1999 May 21;288(5):911-40 PMID: 10329189
  19. Identification of a cis-acting dendritic targeting element in MAP2 mRNAs.
    J Neurosci. 1999 Oct 15;19(20):8818-29 PMID: 10516301
  20. Clonal analysis of Drosophila embryonic neuroblasts: neural cell types, axon projections and muscle targets.
    Development. 1999 Nov;126(21):4653-89 PMID: 10518486
  21. The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation.
    Dev Biol. 1999 Dec 15;216(2):671-80 PMID: 10642801
  22. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.
    Nature. 2000 Feb 24;403(6772):901-6 PMID: 10706289
  23. EMBOSS: the European Molecular Biology Open Software Suite.
    Trends Genet. 2000 Jun;16(6):276-7 PMID: 10827456
  24. The lin-41 RBCC gene acts in the C. elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor.
    Mol Cell. 2000 Apr;5(4):659-69 PMID: 10882102
  25. CaMKIIalpha 3' untranslated region-directed mRNA translocation in living neurons: visualization by GFP linkage.
    J Neurosci. 2000 Sep 1;20(17):6385-93 PMID: 10964944
  26. Two cis-acting elements in the 3' untranslated region of alpha-CaMKII regulate its dendritic targeting.
    Nat Neurosci. 2000 Nov;3(11):1079-84 PMID: 11036263
  27. Protein synthesis at synaptic sites on dendrites.
    Annu Rev Neurosci. 2001;24:299-325 PMID: 11283313
  28. Dynamic visualization of local protein synthesis in hippocampal neurons.
    Neuron. 2001 May;30(2):489-502 PMID: 11395009
  29. A cellular mechanism for targeting newly synthesized mRNAs to synaptic sites on dendrites.
    Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7062-8 PMID: 11416188
  30. Local translation of classes of mRNAs that are targeted to neuronal dendrites.
    Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7080-5 PMID: 11416191
  31. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.
    Cell. 2001 Jul 13;106(1):23-34 PMID: 11461699
  32. Drosophila neuroblasts sequentially express transcription factors which specify the temporal identity of their neuronal progeny.
    Cell. 2001 Aug 24;106(4):511-21 PMID: 11525736
  33. Control of developmental timing in animals.
    Nat Rev Genet. 2001 Sep;2(9):690-701 PMID: 11533718
  34. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans.
    Genes Dev. 2001 Oct 15;15(20):2654-9 PMID: 11641272
  35. Identification of novel genes coding for small expressed RNAs.
    Science. 2001 Oct 26;294(5543):853-8 PMID: 11679670
  36. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.
    Science. 2001 Oct 26;294(5543):858-62 PMID: 11679671
  37. An extensive class of small RNAs in Caenorhabditis elegans.
    Science. 2001 Oct 26;294(5543):862-4 PMID: 11679672
  38. Temporal coordinates: the genes that fix cell fate with birth order.
    Dev Cell. 2001 Sep;1(3):313-4 PMID: 11702940
  39. Neuronal RNA granules: a link between RNA localization and stimulation-dependent translation.
    Neuron. 2001 Nov 20;32(4):683-96 PMID: 11719208
  40. Localization and translation of mRNA in dendrites and axons.
    Nat Rev Neurosci. 2001 Dec;2(12):889-98 PMID: 11733796
  41. Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation.
    Neuron. 2001 Dec 20;32(6):1013-26 PMID: 11754834
  42. miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.
    Genes Dev. 2002 Mar 15;16(6):720-8 PMID: 11914277
  43. The message and the messenger: delivering RNA in neurons.
    Sci STKE. 2002 Apr 2;2002(126):pe16 PMID: 11930084
  44. Identification of tissue-specific microRNAs from mouse.
    Curr Biol. 2002 Apr 30;12(9):735-9 PMID: 12007417
  45. MicroRNAs in plants.
    Genes Dev. 2002 Jul 1;16(13):1616-26 PMID: 12101121
  46. Endogenous and silencing-associated small RNAs in plants.
    Plant Cell. 2002 Jul;14(7):1605-19 PMID: 12119378
  47. Axonal protein synthesis provides a mechanism for localized regulation at an intermediate target.
    Cell. 2002 Jul 26;110(2):223-35 PMID: 12150930
  48. Prediction of plant microRNA targets.
    Cell. 2002 Aug 23;110(4):513-20 PMID: 12202040
  49. Small RNAs correspond to centromere heterochromatic repeats.
    Science. 2002 Sep 13;297(5588):1831 PMID: 12193644
  50. Translating axon guidance cues.
    Cell. 2002 Sep 6;110(5):537-40 PMID: 12230970
  51. Identification of mRNA/protein (mRNP) complexes containing Puralpha, mStaufen, fragile X protein, and myosin Va and their association with rough endoplasmic reticulum equipped with a kinesin motor.
    J Biol Chem. 2002 Oct 4;277(40):37804-10 PMID: 12147688
  52. Synaptic tagging -- who's it?
    Nat Rev Neurosci. 2002 Oct;3(10):813-20 PMID: 12360325
  53. Control of developmental timing by micrornas and their targets.
    Annu Rev Cell Dev Biol. 2002;18:495-513 PMID: 12142272
  54. Fragile X-related protein and VIG associate with the RNA interference machinery.
    Genes Dev. 2002 Oct 1;16(19):2491-6 PMID: 12368260
  55. Regulation and function of local protein synthesis in neuronal dendrites.
    Trends Biochem Sci. 2002 Oct;27(10):506-13 PMID: 12368086
  56. A receptor for activated C kinase is part of messenger ribonucleoprotein complexes associated with polyA-mRNAs in neurons.
    J Neurosci. 2002 Oct 15;22(20):8827-37 PMID: 12388589
  57. The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis.
    Genes Dev. 2002 Nov 1;16(21):2733-42 PMID: 12414724
  58. Transport of fragile X mental retardation protein via granules in neurites of PC12 cells.
    Mol Cell Biol. 2002 Dec;22(23):8332-41 PMID: 12417734
  59. DICER-LIKE1: blind men and elephants in Arabidopsis development.
    Trends Plant Sci. 2002 Nov;7(11):487-91 PMID: 12417148
  60. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA.
    Science. 2001 Aug 3;293(5531):834-8 PMID: 11452083
  61. Localized synaptic potentiation by BDNF requires local protein synthesis in the developing axon.
    Neuron. 2002 Nov 14;36(4):675-88 PMID: 12441056
  62. RNA interference machinery regulates chromosome dynamics during mitosis and meiosis in fission yeast.
    Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):193-8 PMID: 12509501
  63. New microRNAs from mouse and human.
    RNA. 2003 Feb;9(2):175-9 PMID: 12554859
  64. Numerous microRNPs in neuronal cells containing novel microRNAs.
    RNA. 2003 Feb;9(2):180-6 PMID: 12554860
  65. Vertebrate microRNA genes.
    Science. 2003 Mar 7;299(5612):1540 PMID: 12624257
  66. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans.
    Cell. 1993 Dec 3;75(5):855-62 PMID: 8252622
  67. Neuronal polarity.
    Annu Rev Neurosci. 1994;17:267-310 PMID: 8210176
  68. Synaptogenesis in hippocampal cultures: evidence indicating that axons and dendrites become competent to form synapses at different stages of neuronal development.
    J Neurosci. 1994 Nov;14(11 Pt 1):6695-706 PMID: 7965070
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.2333854100
Published
2004-01-06
Epub
2003-00-22
Pages
360-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC314190
Subset
IM
Grants
NIGMS NIH HHS · R01 GM044619 · United States
NIGMS NIH HHS · GM44619 · United States
External Links
PubMed source DOI: 10.1073/pnas.2333854100 PMC Full Text View journal details
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