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PMID: 16533950 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane.

The Journal of cell biology ·Vol. 172 ·No. 6 ·2006-03-13 ·Pages 923-35

Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ

Abstract

Exosomes are secreted, single membrane organelles of approximately 100 nm diameter. Their biogenesis is typically thought to occur in a two-step process involving (1) outward vesicle budding at limiting membranes of endosomes (outward = away from the cytoplasm), which generates intralumenal vesicles, followed by (2) endosome-plasma membrane fusion, which releases these internal vesicles into the extracellular milieu as exosomes. In this study, we present evidence that certain cells, including Jurkat T cells, possess discrete domains of plasma membrane that are enriched for exosomal and endosomal proteins, retain the endosomal property of outward vesicle budding, and serve as sites of immediate exosome biogenesis. It has been hypothesized that retroviruses utilize the exosome biogenesis pathway for the formation of infectious particles. In support of this, we find that Jurkat T cells direct the key budding factor of HIV, HIV Gag, to these endosome-like domains of plasma membrane and secrete HIV Gag from the cell in exosomes.

MeSH Terms
Antigens, CD/metabolism Cell Membrane/metabolism,ultrastructure Cell Surface Extensions/metabolism,ultrastructure Endosomes/metabolism Exocytosis/physiology Gene Products, gag/metabolism HIV Infections/metabolism HIV-1/metabolism,ultrastructure Humans Jurkat Cells Membrane Lipids/metabolism Microscopy, Electron, Transmission Organelles/metabolism Peptide Fragments/metabolism Platelet Membrane Glycoproteins/metabolism T-Lymphocytes/metabolism,ultrastructure,virology Tetraspanin 28 Tetraspanin 30 Transport Vesicles/metabolism,ultrastructure Virion/metabolism,ultrastructure Virus Replication/physiology gag Gene Products, Human Immunodeficiency Virus
Chemicals
Antigens, CD CD63 protein, human CD81 protein, human Gene Products, gag Membrane Lipids Peptide Fragments Platelet Membrane Glycoproteins Tetraspanin 28 Tetraspanin 30 gag Gene Products, Human Immunodeficiency Virus p2 gag peptide, Human immunodeficiency virus 1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Booth Amy M
Department of Biological Chemistry and 2Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Fang Yi
Fallon Jonathan K
Yang Jr-Ming
Hildreth James E K
Gould Stephen J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200508014
Published
2006-03-13
Pages
923-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2063735
Subset
IM
Grants
NIDDK NIH HHS · R01 DK045787 · United States
NIAID NIH HHS · R21 AI062479 · United States
NIAID NIH HHS · AI62479 · United States
NIDDK NIH HHS · DK45787 · United States
External Links
PubMed source DOI: 10.1083/jcb.200508014 PMC Full Text View journal details
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