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PMID: 20818374 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oligomeric organization of the B-cell antigen receptor on resting cells.

Nature ·Vol. 467 ·No. 7314 ·2010-09-23 ·Pages 465-9

Yang J, Reth M

Abstract

B lymphocytes are activated by many different antigens to produce specific antibodies protecting higher organisms from infection. To detect its cognate antigen, each B cell contains up to 120,000 B-cell antigen receptor (BCR) complexes on its cell surface. How these abundant receptors stay silent on resting B cells and how they can be activated by a molecularly diverse set of ligands is poorly understood. Here we show, with the use of a quantitative bifluorescence complementation assay (BiFC), that the BCR has an intrinsic ability to form oligomers on the surface of living cells. A BCR mutant that fails to form oligomers is more active and cannot be expressed stably on the B-cell surface, whereas BiFC-stabilized BCR oligomers are less active and more strongly expressed on the surface. We propose that oligomers are the autoinhibited form of the BCR and that it is the shift from closed BCR oligomers to clustered monomers that drives B-cell activation in a way that is independent of the structural input from the antigen.

MeSH Terms
Animals B-Lymphocytes/cytology,immunology,metabolism Cell Line Drosophila melanogaster/cytology Immunoglobulin D/genetics,immunology Lymphocyte Activation/immunology Protein Multimerization/immunology Receptors, Antigen, B-Cell/antagonists & inhibitors,chemistry,immunology,metabolism
Chemicals
Immunoglobulin D Receptors, Antigen, B-Cell
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yang Jianying
Centre for Biological Signalling Studies BIOSS, Albert-Ludwigs-Universität Freiburg, Department of Molecular Immunology, Faculty of Biology, Albert-Ludwigs-Universität Freiburg and Max Planck Institute for Immunobiology, Stübeweg 51, 79108 Freiburg, Germany.
Reth Michael
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
DOI
10.1038/nature09357
Published
2010-09-23
Epub
2010-00-05
Pages
465-9
Language
English
Region
England
NLM ID
0410462
Subset
IM
External Links
PubMed source DOI: 10.1038/nature09357 View journal details
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