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PMID: 18573091 Published · ppublish English Journal Article Review

Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells.

Annual review of biophysics ·Vol. 37 ·2008-00-00 ·Pages 465-87

Kerppola TK

Abstract

Protein interactions are a fundamental mechanism for the generation of biological regulatory specificity. The study of protein interactions in living cells is of particular significance because the interactions that occur in a particular cell depend on the full complement of proteins present in the cell and the external stimuli that influence the cell. Bimolecular fluorescence complementation (BiFC) analysis enables direct visualization of protein interactions in living cells. The BiFC assay is based on the association between two nonfluorescent fragments of a fluorescent protein when they are brought in proximity to each other by an interaction between proteins fused to the fragments. Numerous protein interactions have been visualized using the BiFC assay in many different cell types and organisms. The BiFC assay is technically straightforward and can be performed using standard molecular biology and cell culture reagents and a regular fluorescence microscope or flow cytometer.

MeSH Terms
Cell Physiological Phenomena Microscopy, Fluorescence/methods Molecular Probe Techniques Protein Interaction Mapping/methods Proteome/metabolism Signal Transduction/physiology
Chemicals
Proteome
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kerppola Tom K
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA. kerppola@umich.edu
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Article Info
Journal
Annual review of biophysics
Abbr.
Annu Rev Biophys
ISSN
1936-122X
DOI
10.1146/annurev.biophys.37.032807.125842
Email
kerppola@umich.edu
Published
2008-00-00
Pages
465-87
Language
English
Region
United States
NLM ID
101469708
PMCID
PMC2829326
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01 GM086213 · United States
NIGMS NIH HHS · R01 GM086213-01 · United States
External Links
PubMed source DOI: 10.1146/annurev.biophys.37.032807.125842 PMC Full Text View journal details
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