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PMID: 15972641 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

Independent trafficking of Ig-alpha/Ig-beta and mu-heavy chain is facilitated by dissociation of the B cell antigen receptor complex.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 175 ·No. 1 ·2005-07-01 ·Pages 147-54

Kim JH, Cramer L, Mueller H, Wilson B, Vilen BJ

Abstract

The BCR relays extracellular signals and internalizes Ag for processing and presentation. We have previously demonstrated that ligation of the BCR destabilizes Ig-alpha/Ig-beta (Ig-alphabeta) from mu-H chain (mum). In this study we report that receptor destabilization represents a physical separation of mum from Ig-alphabeta. Sucrose gradient fractionation localized Ig-alphabeta to G(M1)-containing lipid microdomains in the absence of mum. Confocal and electron microscopy studies revealed the colocalization of unsheathed mum with clathrin-coated vesicles. Furthermore, mum failed to associate with clathrin-coated vesicles when receptor destabilization was inhibited, suggesting that unsheathing of mum is required for clathrin-mediated endocytosis. In summary, we found that Ag stimulation physically separates Ig-alphabeta from mum, facilitating concomitant signal transduction and Ag delivery to the endocytic compartment.

MeSH Terms
Animals Antigen Presentation Biological Transport, Active Cell Line Clathrin-Coated Vesicles/immunology,metabolism Endocytosis G(M1) Ganglioside/metabolism Immunoglobulin Heavy Chains/metabolism Immunoglobulin alpha-Chains/metabolism Immunoglobulin mu-Chains/metabolism Kinetics Membrane Microdomains/immunology,metabolism Mice Microscopy, Electron Receptors, Antigen, B-Cell/metabolism Signal Transduction
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin alpha-Chains Immunoglobulin mu-Chains Receptors, Antigen, B-Cell G(M1) Ganglioside
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kim Jin-Hyang
Department of Pathology and the Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA.
Cramer Lorraine
Mueller Heather
Wilson Bridget
Vilen Barbara J
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Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
0022-1767
DOI
10.4049/jimmunol.175.1.147
Published
2005-07-01
Pages
147-54
Language
English
Region
United States
NLM ID
2985117R
PMCID
PMC3895480
Subset
IM
Grants
NIAID NIH HHS · R01 AI053266 · United States
External Links
PubMed source DOI: 10.4049/jimmunol.175.1.147 PMC Full Text View journal details
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