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PMID: 2072910 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure and transforming potential of the human cot oncogene encoding a putative protein kinase.

Molecular and cellular biology ·Vol. 11 ·No. 8 ·1991-08-00 ·Pages 4088-96

Miyoshi J, Higashi T, Mukai H, Ohuchi T, Kakunaga T

Abstract

A new transforming gene has been molecularly cloned from hamster SHOK cells transformed with DNA extracted from a human thyroid carcinoma cell line and named the cot (cancer Osaka thyroid) oncogene. cDNA sequencing disclosed that this oncogene codes for a protein with 415 amino acid residues, and computer matching showed 42 to 48% similarity matches with serine protein kinases. Its gene product was identified as a 52-kDa protein by transcription and translation in vitro. Expression of cot cDNA under transcriptional control by a retroviral long terminal repeat induced morphological transformation of NIH 3T3 cells as well as SHOK cells. Protein kinase activity associated with constructed p60gag-cot was detected by immune complex kinase assay with anti-gag antiserum. The cot oncogene was overexpressed in transformed SHOK cells and found to have a rearranged 3' end in the last coding exon, which probably resulted in a deletion and an altered C' terminus in the transforming protein. This DNA rearrangement appeared to have occurred during transfection of the tumor DNA into hamster SHOK cells and not in the original thyroid tumor.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cell Transformation, Neoplastic Exons Gene Rearrangement Humans MAP Kinase Kinase Kinases Mice Molecular Sequence Data Multigene Family Oncogene Proteins Oncogenes Plasmids Protein Kinases/genetics Proto-Oncogene Proteins Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Transfection
Chemicals
Oncogene Proteins Proto-Oncogene Proteins Protein Kinases MAP Kinase Kinase Kinases MAP3K8 protein, human
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Miyoshi J
Department of Oncogene Research, Osaka University, Japan.
Higashi T
Mukai H
Ohuchi T
Kakunaga T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.8.4088-4096.1991
Published
1991-08-00
Pages
4088-96
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361219
Subset
IM
Databases
GENBANK
D00651, D00652, D00653, D00654, M64825, M64826, M64827, S43246, S43885, S43914
External Links
PubMed source DOI: 10.1128/mcb.11.8.4088-4096.1991 PMC Full Text View journal details
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