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PMID: 2050120 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A pause site for RNA polymerase II is associated with termination of transcription.

The EMBO journal ·Vol. 10 ·No. 7 ·1991-07-00 ·Pages 1833-42

Enriquez-Harris P, Levitt N, Briggs D, Proudfoot NJ

Abstract

Termination of transcription by RNA polymerase II has been postulated to involve a pausing process. We have identified such a pause signal, 350 bp into the 3' flanking region of the human alpha 2 globin gene at a position where termination is thought to occur. We show that this pause signal enhances the utilization of an upstream poly(A) site which is otherwise out-competed by a stronger downstream poly(A) site. We also demonstrate that the pause site rescues a poly(A) site that is inactive due to its location within an intron. Using nuclear run-on analysis we show that elongating RNA polymerase II molecules accumulate over this pause signal. Furthermore we show that when the pause site is positioned immediately downstream of a strong poly(A) signal, significant levels of transcription termination take place.

MeSH Terms
Base Sequence Gene Expression Regulation Globins/genetics HeLa Cells Humans Introns Molecular Sequence Data Poly A/genetics RNA Polymerase II/genetics,metabolism Terminator Regions, Genetic Transcription, Genetic
Chemicals
Poly A Globins RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Enriquez-Harris P
Sir William Dunn School of Pathology, University of Oxford, UK.
Levitt N
Briggs D
Proudfoot N J
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42 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-07-00
Pages
1833-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452858
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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