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PMID: 3025624 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tripartite sequences within and 3' to the sea urchin H2A histone gene display properties associated with a transcriptional termination process.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 4008-18

Johnson MR, Norman C, Reeve MA, Scully J, Proudfoot NJ

Abstract

We have defined a DNA sequence that behaves as an RNA polymerase II termination signal by using the human HeLa cell transient expression system. Surprisingly, this sequence is tripartite, including part of the coding region of the sea urchin H2A histone gene together with two separate sequences in the 3' flanking region of the gene. We demonstrate that this signal functions both in its normal gene environment and also when placed within the human alpha-globin gene. However, we have failed to detect a discrete 3' terminus. Rather, our data indicate the presence of an extremely heterogeneous series of nonpolyadenylated RNAs. These heterogeneous nonpolyadenylated RNAs are stable when transcribed from the intact histone gene but are highly unstable within the human alpha-globin gene. This provides evidence for the role of poly(A) in the stability of mRNA.

MeSH Terms
Animals Base Sequence Chromosome Deletion DNA Restriction Enzymes Genes HeLa Cells/metabolism Histones/genetics Humans Plasmids Sea Urchins/genetics Transcription, Genetic
Chemicals
Histones DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Johnson M R
Norman C
Reeve M A
Scully J
Proudfoot N J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.4008-4018.1986
Published
1986-11-00
Pages
4008-18
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367165
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.11.4008-4018.1986 PMC Full Text View journal details
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