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PMID: 20473271 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion.

The EMBO journal ·Vol. 29 ·No. 12 ·2010-06-16 ·Pages 1948-60

Xu H, Jun Y, Thompson J, Yates J, Wickner W

Abstract

SNARE-dependent membrane fusion requires the disassembly of cis-SNARE complexes (formed by SNAREs anchored to one membrane) followed by the assembly of trans-SNARE complexes (SNAREs anchored to two apposed membranes). Although SNARE complex disassembly and assembly might be thought to be opposing reactions, the proteins promoting disassembly (Sec17p/Sec18p) and assembly (the HOPS complex) work synergistically to support fusion. We now report that trans-SNARE complexes formed during vacuole fusion are largely associated with Sec17p. Using a reconstituted proteoliposome fusion system, we show that trans-SNARE complex, like cis-SNARE complex, is sensitive to Sec17p/Sec18p mediated disassembly. Strikingly, HOPS inhibits the disassembly of SNARE complexes in the trans-, but not in the cis-, configuration. This selective HOPS preservation of trans-SNARE complexes requires HOPS:SNARE recognition and is lost when the apposed bilayers are dissolved in Triton X-100; it is also observed during fusion of isolated vacuoles. HOPS thus directs the Sec17p/Sec18p chaperone system to maximize functional trans-SNARE complex for membrane fusion, a new role of tethering factors during membrane traffic.

MeSH Terms
Adenosine Triphosphatases/metabolism Cell Membrane/metabolism Membrane Fusion Models, Biological SNARE Proteins/metabolism Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins/metabolism Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins/metabolism Vacuoles/metabolism Vesicular Transport Proteins/metabolism
Chemicals
SEC17 protein, S cerevisiae SNARE Proteins Saccharomyces cerevisiae Proteins Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Vesicular Transport Proteins Adenosine Triphosphatases SEC18 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Xu Hao
Department of Biochemistry, Dartmouth Medical School, Hanover, NH, USA.
Jun Youngsoo
Thompson James
Yates John
Wickner William
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2010.97
Published
2010-06-16
Epub
2010-00-14
Pages
1948-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC2892374
Subset
IM
Grants
NCRR NIH HHS · P41 RR011823 · United States
NIGMS NIH HHS · R01 GM023377 · United States
NCRR NIH HHS · P41 RR11823 · United States
NIGMS NIH HHS · GM23377 · United States
External Links
PubMed source DOI: 10.1038/emboj.2010.97 PMC Full Text View journal details
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