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PMID: 10022848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis.

The EMBO journal ·Vol. 18 ·No. 4 ·1999-02-15 ·Pages 1071-80

Guo W, Roth D, Walch-Solimena C, Novick P

Abstract

Polarized secretion requires proper targeting of secretory vesicles to specific sites on the plasma membrane. Here we report that the exocyst complex plays a key role in vesicle targeting. Sec15p, an exocyst component, can associate with secretory vesicles and interact specifically with the rab GTPase, Sec4p, in its GTP-bound form. A chain of protein-protein interactions leads from Sec4p and Sec15p on the vesicle, through various subunits of the exocyst, to Sec3p, which marks the sites of exocytosis on the plasma membrane. Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion.

MeSH Terms
Cell Membrane/metabolism Centrifugation, Density Gradient Cytoplasmic Granules/metabolism Exocytosis Fungal Proteins/metabolism GTP-Binding Proteins/metabolism Microscopy, Immunoelectron Mutation Precipitin Tests Protein Binding Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Vesicular Transport Proteins rab GTP-Binding Proteins
Chemicals
Fungal Proteins SEC10 protein, S cerevisiae SEC15 protein, S cerevisiae SEC3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Vesicular Transport Proteins GTP-Binding Proteins SEC4 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guo W
Department of Cell Biology, Yale University School of Medicine, PO Box 208002, New Haven, CT 06520-8002, USA.
Roth D
Walch-Solimena C
Novick P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.4.1071
Published
1999-02-15
Pages
1071-80
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171198
Subset
IM
Grants
NIGMS NIH HHS · GM-35370 · United States
External Links
PubMed source DOI: 10.1093/emboj/18.4.1071 PMC Full Text View journal details
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