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PMID: 18007597 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion.

The EMBO journal ·Vol. 26 ·No. 24 ·2007-12-12 ·Pages 4935-45

Jun Y, Xu H, Thorngren N, Wickner W

Abstract

Intracellular membrane fusion requires SNARE proteins in a trans-complex, anchored to apposed membranes. Proteoliposome studies have suggested that SNAREs drive fusion by stressing the lipid bilayer via their transmembrane domains (TMDs), and that SNARE complexes require a TMD in each docked membrane to promote fusion. Yeast vacuole fusion is believed to require three Q-SNAREs from one vacuole and the R-SNARE Nyv1p from its fusion partner. In accord with this model, we find that fusion is abolished when the TMD of Nyv1p is replaced by lipid anchors, even though lipid-anchored Nyv1p assembles into trans-SNARE complexes. However, normal fusion is restored by the addition of both Sec18p and the soluble SNARE Vam7p. In restoring fusion, Sec18p promotes the disassembly of trans-SNARE complexes, and Vam7p enhances their assembly. Thus, either the TMD of this R-SNARE is not essential for fusion, and TMD-mediated membrane stress is not the only mode of trans-SNARE complex action, or these SNAREs have more flexibility than heretofore appreciated to form alternate functional complexes that violate the 3Q:1R rule.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Lipid Bilayers/chemistry,metabolism Membrane Fusion/physiology Membrane Lipids/chemistry Membrane Proteins/genetics,metabolism Multiprotein Complexes/metabolism Protein Conformation Protein Structure, Tertiary Qa-SNARE Proteins/genetics,metabolism Qc-SNARE Proteins/genetics,metabolism R-SNARE Proteins/chemistry,genetics,metabolism Recombinant Fusion Proteins/chemistry,genetics,metabolism SNARE Proteins/chemistry,genetics,metabolism Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Synaptosomal-Associated Protein 25 Vacuoles/metabolism Vesicular Transport Proteins/genetics,metabolism
Chemicals
Lipid Bilayers Membrane Lipids Membrane Proteins Multiprotein Complexes Nyv1 protein, S cerevisiae Qa-SNARE Proteins Qc-SNARE Proteins R-SNARE Proteins Recombinant Fusion Proteins SNARE Proteins Saccharomyces cerevisiae Proteins Synaptosomal-Associated Protein 25 VAM3 protein, S cerevisiae VAM7 protein, S cerevisiae Vesicular Transport Proteins Adenosine Triphosphatases SEC18 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jun Youngsoo
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755-3844, USA.
Xu Hao
Thorngren Naomi
Wickner William
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/sj.emboj.7601915
Published
2007-12-12
Epub
2007-00-15
Pages
4935-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC2140102
Subset
IM
Grants
NIGMS NIH HHS · R01 GM023377 · United States
NIGMS NIH HHS · GM23377 · United States
External Links
PubMed source DOI: 10.1038/sj.emboj.7601915 PMC Full Text View journal details
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