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PMID: 16027221 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

SNAREs can promote complete fusion and hemifusion as alternative outcomes.

The Journal of cell biology ·Vol. 170 ·No. 2 ·2005-07-18 ·Pages 249-60

Giraudo CG, Hu C, You D, Slovic AM, Mosharov EV, Sulzer D, Melia TJ, Rothman JE

Abstract

Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one third were reversible. We predict that this relatively close balance among outcomes could be tipped by binding of regulatory proteins to the SNAREs, allowing for dynamic physiological regulation between full fusion and reversible kiss-and-run-like events.

MeSH Terms
3T3 Cells Animals CHO Cells Cell Membrane/physiology Cricetinae Cricetulus Membrane Fusion/genetics,physiology Mice Protein Conformation Recombinant Proteins/genetics,metabolism SNARE Proteins Vesicular Transport Proteins/chemistry,genetics,metabolism
Chemicals
Recombinant Proteins SNARE Proteins Vesicular Transport Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Giraudo Claudio G
Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Hu Chuan
You Daoqi
Slovic Avram M
Mosharov Eugene V
Sulzer David
Melia Thomas J
Rothman James E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200501093
Published
2005-07-18
Pages
249-60
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171417
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200501093 PMC Full Text View journal details
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