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PMID: 20467781 Published · ppublish English Journal Article Review

Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters.

Journal of industrial microbiology & biotechnology ·Vol. 37 ·No. 8 ·2010-08-00 ·Pages 759-72

Baltz RH

Abstract

Natural products discovery from actinomycetes has been on the decline in recent years, and has suffered from a lack of innovative ways to discover new secondary metabolites within a background of the thousands of known compounds. Recent advances in whole genome sequencing have revealed that actinomycetes with large genomes encode multiple secondary metabolite pathways, most of which remain cryptic. One approach to address the expression of cryptic pathways is to first identify novel pathways by bioinformatics, then clone and express them in well-characterized hosts with known secondary metabolomes. This process should eliminate the tedious dereplication process that has hampered natural products discovery. Several laboratory and industrial production strains have been used for heterologous production of secondary metabolite pathways. This review discusses the results of these studies, and the pros and cons of using various Streptomyces and one Saccharopolyspora strain for heterologous expression. This information should provide an experimental basis to help researchers choose hosts for current application and future development to express heterologous secondary metabolite pathways in yields sufficient for rapid scale-up, biological testing, and commercial production.

MeSH Terms
Actinobacteria/genetics Cloning, Molecular Gene Expression Industrial Microbiology Metabolic Networks and Pathways Multigene Family Recombinant Proteins/biosynthesis,genetics Saccharopolyspora/genetics,metabolism Streptomyces/genetics,metabolism
Chemicals
Recombinant Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Baltz Richard H
CognoGen Biotechnology Consulting, 6438 North Olney Street, Indianapolis, IN 46220, USA. rbaltz923@aol.com
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Article Info
Journal
Journal of industrial microbiology & biotechnology
Abbr.
J Ind Microbiol Biotechnol
ISSN
1476-5535
DOI
10.1007/s10295-010-0730-9
Email
rbaltz923@aol.com
Published
2010-08-00
Epub
2010-00-14
Pages
759-72
Language
English
Region
Germany
NLM ID
9705544
Subset
IM
External Links
PubMed source DOI: 10.1007/s10295-010-0730-9 View journal details
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