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PMID: 20430825 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation.

Nucleic acids research ·Vol. 38 ·No. 16 ·2010-09-00 ·Pages 5554-68

Uchiumi T, Ohgaki K, Yagi M, Aoki Y, Sakai A, Matsumoto S, Kang D

Abstract

ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. Depletion of prokaryotic Era inhibits cell division without affecting chromosome segregation. Previously, we isolated ERAL1 protein as one of proteins which were associated with mitochondrial transcription factor A by using immunoprecipitation. In this study, we analysed the localization and function of ERAL1 in mammalian cells. ERAL1 was localized in mitochondrial matrix and associated with mitoribosomal proteins including the 12S rRNA. siRNA knockdown of ERAL1 decreased mitochondrial translation, caused redistribution of ribosomal small subunits and reduced 12S rRNA. The knockdown of ERAL1 in human HeLa cells elevated mitochondrial superoxide production and slightly decreased mitochondrial membrane potential. The knockdown inhibited the growth of HeLa cells with an accumulation of apoptotic cells. These results suggest that ERAL1 is localized in a small subunit of the mitochondrial ribosome, plays an important role in the small ribosomal constitution, and is also involved in cell viability.

MeSH Terms
Apoptosis Cell Proliferation Electron Transport Complex IV/metabolism GTP-Binding Proteins/analysis,antagonists & inhibitors,physiology HeLa Cells Humans Immunoprecipitation Membrane Potential, Mitochondrial Mitochondria/genetics,metabolism Mitochondrial Proteins/analysis,antagonists & inhibitors,physiology Protein Biosynthesis RNA/isolation & purification RNA, Mitochondrial RNA-Binding Proteins/analysis,antagonists & inhibitors,physiology Reactive Oxygen Species Ribosome Subunits, Small, Eukaryotic/metabolism
Chemicals
Mitochondrial Proteins RNA, Mitochondrial RNA-Binding Proteins Reactive Oxygen Species RNA Electron Transport Complex IV ERAL1 protein, human GTP-Binding Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Uchiumi Takeshi
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. uchiumi@cclm.med.kyushu-u.ac.jp
Ohgaki Kippei
Yagi Mikako
Aoki Yoshimasa
Sakai Aya
Matsumoto Shinya
Kang Dongchon
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkq305
Email
uchiumi@cclm.med.kyushu-u.ac.jp
Published
2010-09-00
Epub
2010-00-29
Pages
5554-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2938226
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkq305 PMC Full Text View journal details
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