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PMID: 2023921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

c-rel activates but v-rel suppresses transcription from kappa B sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3715-9

Inoue J, Kerr LD, Ransone LJ, Bengal E, Hunter T, Verma IM

Abstract

We show that the product of the protooncogene c-rel is a constituent of an NF-kappa B-like complex that binds to the kappa B site originally identified in the enhancer of immunoglobulin kappa light chain gene. c-rel protein synthesized in bacteria binds to the kappa B site in a sequence-specific manner. The rel-kappa B complex can be disrupted by incubation with anti-rel antibodies. The rel protein can form oligomers. The c-rel protein can activate transcription from promoters containing kappa B sites; v-rel, on the other hand, suppresses the transcription of genes linked to kappa B sites. Thus, v-rel may interfere with the normal transcriptional machinery of the cell by acting as a dominant negative mutant.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Gene Expression Regulation In Vitro Techniques Mice Molecular Sequence Data NF-kappa B/physiology Oncogene Proteins v-rel Proto-Oncogene Proteins/physiology Proto-Oncogene Proteins c-rel Recombinant Proteins Regulatory Sequences, Nucleic Acid Repressor Proteins/physiology Retroviridae Proteins, Oncogenic/physiology Transcription Factors/physiology Transcription, Genetic
Chemicals
NF-kappa B Oncogene Proteins v-rel Proto-Oncogene Proteins Proto-Oncogene Proteins c-rel Recombinant Proteins Repressor Proteins Retroviridae Proteins, Oncogenic Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Inoue J
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92186-5800.
Kerr L D
Ransone L J
Bengal E
Hunter T
Verma I M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.3715
Published
1991-05-01
Pages
3715-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51523
Subset
IM
Grants
NCI NIH HHS · 2 T32 CA 09370 · United States
NCI NIH HHS · F2 CA 08585A · United States
FIC NIH HHS · F5TW04430 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.9.3715 PMC Full Text View journal details
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