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PMID: 201607 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli.

Journal of bacteriology ·Vol. 133 ·No. 1 ·1978-01-00 ·Pages 196-202

Iwaya M, Jones CW, Khorana J, Strominger JL

Abstract

Genes responsible for round morphology in mecillinam-resistant, round morphological mutants of Escherichia coli have been mapped. Three mutants, called rodX, mapped at around 14 min, and two, called rodY, mapped at around 70 min by P1 transduction. These are either the same or very close to the loci reported, respectively, for the rodA (H. Matsuzawa, K. Hayakawa, T. Sato, and K. Imahori, J. Bacteriol. 115:436-442, 1973) and envB genes (B. Westling-Häggström and S. Normark, J. Bacteriol. 123:75-82, 1975). This suggests that mecillinam can be used very efficiently to select for found morphological mutants of rodA and envB after nitrosoguanidine treatment.

MeSH Terms
Amdinocillin/pharmacology Chromosome Mapping Escherichia coli/drug effects,genetics Genes Mutation Penicillanic Acid/pharmacology Penicillin Resistance Phenotype Transduction, Genetic
Chemicals
Penicillanic Acid Amdinocillin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Iwaya M
Jones C W
Khorana J
Strominger J L
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.1.196-202.1978
Published
1978-01-00
Pages
196-202
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221994
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.1.196-202.1978 PMC Full Text View journal details
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