Home LiteratureArticle Details
PMID: 4196251 Published · ppublish English Journal Article

Escherichia coli mutants permissive for T4 bacteriophage with deletion in e gene (phage lysozyme).

Journal of bacteriology ·Vol. 114 ·No. 2 ·1973-05-00 ·Pages 656-65

Sundar Raj CV, Wu HC

Abstract

Escherichia coli mutants have been isolated that are permissive for the infection by T4 phage with deletion in the cistron for the phage lysozyme, the e gene. Some, but not all, of these mutants are simultaneously permissive for the infection by T4 phage defective in the t gene, the product of which has also been implicated in the release of progeny phages. Most of these mutants shared the following properties: temperature sensitivity in growth and cell division, increased sensitivity towards a number of unrelated antibiotics and colicins, and increased sensitivity towards anionic detergents (sodium dodecyl sulfate and sodium deoxycholate). The possible biochemical basis for these phenotypes is discussed.

MeSH Terms
Anti-Bacterial Agents/pharmacology Cell Division Colicins/pharmacology Coliphages/enzymology,growth & development DNA Viruses/enzymology,growth & development Drug Resistance, Microbial Edetic Acid/pharmacology Escherichia coli/drug effects,enzymology,growth & development Genes Glucose-6-Phosphate Isomerase/metabolism Lysogeny Muramidase/biosynthesis,pharmacology Mutagens Mutation Nitrosoguanidines Ribonucleases/metabolism Surface-Active Agents/pharmacology Temperature Tritium
Chemicals
Anti-Bacterial Agents Colicins Mutagens Nitrosoguanidines Surface-Active Agents Tritium Edetic Acid Ribonucleases Muramidase Glucose-6-Phosphate Isomerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sundar Raj C V
Wu H C
References (32)
32 references, click to expand
  1. Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers.
    J Ultrastruct Res. 1967 Jul;19(1):45-83 PMID: 4961452
  2. The mechanism of lysis in phage T4-infected cells.
    Virology. 1967 Nov;33(3):398-404 PMID: 4863172
  3. Giant cells of Escherichia coli.
    J Bacteriol. 1968 Jan;95(1):139-42 PMID: 4866096
  4. Lysis of T4-infected bacteria in the absence of lysozyme.
    Virology. 1968 May;35(1):158-65 PMID: 4871001
  5. Size restriction on peptide utilization in Escherichia coli.
    J Biol Chem. 1968 Dec 10;243(23):6291-9 PMID: 4881360
  6. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
    Virology. 1969 May;38(1):200-2 PMID: 4891223
  7. Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12.
    Biochem Biophys Res Commun. 1969 Aug 15;36(4):682-9 PMID: 4897411
  8. Properties and characteristics of a bacteriocin from Serratia marcescens.
    J Bacteriol. 1969 Sep;99(3):655-60 PMID: 4905530
  9. The lysis mechanism of phage T4: mutants affecting lysis.
    Virology. 1970 Mar;40(3):719-26 PMID: 5437792
  10. [Isolation of membrane mutants in Escherichia coli].
    C R Acad Sci Hebd Seances Acad Sci D. 1970 May 25;270(21):2591-3 PMID: 4987650
  11. [Investigations on the Typing of salmonella R-forms. IV. Typing of S. minnesota-R-mutants by antibiotics].
    Zentralbl Bakteriol Orig. 1970 Apr;213(3):356-80 PMID: 5456377
  12. Current linkage map of Escherichia coli.
    Bacteriol Rev. 1970 Jun;34(2):155-75 PMID: 4918631
  13. Mutants of Escherichia coli with altered surface properties which are refractory to colicin E2, sensitive to ultraviolet light and which can also show recombination deficiency, abortive growth of bacteriophage lambda and filament formation.
    J Gen Microbiol. 1970 Aug;62(3):371-82 PMID: 4924623
  14. Co-transduction with serB of a pleiotropic mutation affecting colicin E2 refractivity, ultraviolet sensitivity, recombination proficiency and surface properties of Escherichia coli K12.
    J Gen Microbiol. 1970 Aug;62(3):383-98 PMID: 4924624
  15. Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase.
    J Bacteriol. 1971 Feb;105(2):455-66 PMID: 5541523
  16. Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12.
    J Bacteriol. 1971 Mar;105(3):968-75 PMID: 4926688
  17. Physiological studies on the t gene defect in T4-infected Escherichia coli.
    Virology. 1971 Apr;44(1):101-7 PMID: 4937243
  18. Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells.
    Nat New Biol. 1971 Dec 1;234(48):133-7 PMID: 4332178
  19. Endopeptidase activity of phage lamba-endolysin.
    Nat New Biol. 1971 Dec 1;234(48):144-5 PMID: 4943686
  20. Inactivation of ribosomes in vitro by colicin E 3 .
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421-5 PMID: 4944624
  21. Leakage of periplasmic enzymes by mutants of Escherichia coli and Salmonella typhimurium: isolation of "periplasmic leaky" mutants.
    J Bacteriol. 1972 Feb;109(2):520-5 PMID: 4333606
  22. Targets of penicillin action in Escherichia coli.
    Nature. 1972 Feb 25;235(5339):426-9 PMID: 4553687
  23. Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12.
    J Bacteriol. 1972 Jun;110(3):935-44 PMID: 4555418
  24. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  25. Isolation and some properties of cell envelope altered mutants of Escherichia coli.
    J Bacteriol. 1972 Aug;111(2):495-8 PMID: 4340865
  26. Relationship between permeability, cell division, and murein metabolism in a mutant of Escherichia coli.
    J Bacteriol. 1972 Aug;111(2):499-509 PMID: 4262303
  27. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  28. A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes.
    Biochim Biophys Acta. 1962 Jul 2;60:422-4 PMID: 14465346
  29. ON THE MECHANISMS OF ACTION OF COLICINS.
    Ann Inst Pasteur (Paris). 1964 Nov;107:SUPPL:67-73 PMID: 14240526
  30. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA.
    Can J Microbiol. 1965 Jun;11:547-60 PMID: 14346132
  31. Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage lambda.
    Virology. 1956 Aug;2(4):565-74 PMID: 13352781
  32. MUTANT OF LAMBDA BACTERIOPHAGE PRODUCING A THERMOLABILE ENDOLYSIN.
    J Bacteriol. 1963 Jun;85:1202-7 PMID: 14047209
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.114.2.656-665.1973
Published
1973-05-00
Pages
656-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251823
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.114.2.656-665.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2