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PMID: 19841138 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis.

The Journal of cell biology ·Vol. 187 ·No. 2 ·2009-10-19 ·Pages 247-64

Delevoye C, Hurbain I, Tenza D, Sibarita JB, Uzan-Gafsou S, Ohno H, Geerts WJ, Verkleij AJ, Salamero J, Marks MS, Raposo G

Abstract

Specialized cell types exploit endosomal trafficking to deliver protein cargoes to cell type-specific lysosome-related organelles (LROs), but how endosomes are specified for this function is not known. In this study, we show that the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes required for cargo delivery to maturing melanosomes. In cells depleted of AP-1 or KIF13A, a subpopulation of recycling endosomes redistributes to pericentriolar clusters, resulting in sequestration of melanosomal enzymes like Tyrp1 in vacuolar endosomes and consequent inhibition of melanin synthesis and melanosome maturation. Immunocytochemistry, live cell imaging, and electron tomography reveal AP-1- and KIF13A-dependent dynamic close appositions and continuities between peripheral endosomal tubules and melanosomes. Our results reveal that LRO protein sorting is coupled to cell type-specific positioning of endosomes that facilitate endosome-LRO contacts and are required for organelle maturation.

MeSH Terms
Cell Line Endosomes/metabolism,ultrastructure Humans Kinesins/genetics,metabolism Melanosomes/metabolism,ultrastructure Microscopy, Electron RNA, Small Interfering/genetics Transcription Factor AP-1/genetics,metabolism
Chemicals
KIF13A protein, human RNA, Small Interfering Transcription Factor AP-1 Kinesins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Delevoye Cédric
Structure and Membrane Compartments, Centre Nationale de la Recherche Scientifique, UMR 144 Institut Curie, Centre de Recherche, Paris F-75248, France.
Hurbain Ilse
Tenza Danièle
Sibarita Jean-Baptiste
Uzan-Gafsou Stéphanie
Ohno Hiroshi
Geerts Willie J C
Verkleij Arie J
Salamero Jean
Marks Michael S
Raposo Graça
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200907122
Published
2009-10-19
Pages
247-64
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2768840
Subset
IM
Grants
NEI NIH HHS · R01 EY015625 · United States
NEI NIH HHS · R01 EY015625-06A1 · United States
Corrections
CommentIn
PMID: 19841135
External Links
PubMed source DOI: 10.1083/jcb.200907122 PMC Full Text View journal details
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