Differential response of the human 6-16 and 9-27 genes to alpha and gamma interferons.

Nucleic acids research ·Vol. 19 ·No. 3 ·1991-02-11 ·Pages 591-8

Ackrill AM, Reid LE, Gilbert CS, Gewert DR, Porter AC, Lewin AR, Stark GR, Kerr IM

Abstract

9-27 mRNA is expressed to a high level in response to both alpha and gamma interferons. In contrast, 6-16 mRNA is expressed well in response to alpha but very poorly in response to gamma interferon in human cells. The factors governing these different levels of expression were investigated. For both genes the major effect of both interferons is on transcription. A transcriptional bias in the 6-16 promoter/enhancer accounts in large part for the differential response of 6-16 to the two interferons. No single DNA element appears responsible; the smaller the 5' region analysed the lower the absolute activity and the smaller the differential response to alpha and gamma interferons observed. Both the 6-16 and 9-27 mRNAs are very stable and no effect of the interferons on stability was detected. Nor was any direct evidence obtained for preferential processing of the 9-27 mRNA. Nevertheless, differentials between the transcription and accumulation of mature mRNAs, particularly for 6-16 mRNA in response to gamma interferon, suggest that post-transcriptional control(s) must additionally operate. The 9-27 5' promoter/enhancer is much less active than that from 6-16 when placed 5' of a marker gene, despite the similar response of the two genes to alpha interferon.

MeSH Terms

Blotting, Northern Cells, Cultured DNA Mutational Analysis Enhancer Elements, Genetic Gene Expression Regulation Genes Humans Interferon Type I/pharmacology Interferon-gamma/pharmacology Metabolic Clearance Rate Multigene Family/genetics Promoter Regions, Genetic RNA Processing, Post-Transcriptional RNA, Messenger/genetics,metabolism Recombinant Proteins Transcription, Genetic/drug effects

Chemicals

Interferon Type I RNA, Messenger Recombinant Proteins Interferon-gamma

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Ackrill A M

Imperial Cancer Research Fund, London, UK.

Reid L E

Gilbert C S

Gewert D R

Porter A C

Lewin A R

Stark G R

Kerr I M

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/19.3.591

Published

1991-02-11

Pages

591-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC333653

Subset

External Links

PubMed source DOI: 10.1093/nar/19.3.591 PMC Full Text View journal details

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Differential response of the human 6-16 and 9-27 genes to alpha and gamma interferons.

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