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PMID: 2498080 Published · ppublish English Journal Article

Induction of assembly of MHC class I heavy chains with beta 2microglobulin by interferon-gamma.

The EMBO journal ·Vol. 8 ·No. 2 ·1989-02-00 ·Pages 475-81

Klar D, Hämmerling GJ

Abstract

Assembly of histocompatibility class I heavy chains with beta 2microglobulin (beta 2m) is known to be necessary for cell surface expression. Studies on the H-2 class I deficient but interferon-gamma (IFN-gamma) inducible fibrosarcoma BC2 and the lung carcinoma CMT 64.5 showed that after transfection with allogeneic H-2 class I genes the class I proteins are expressed, but only intracellularly and not on the cell surface. In spite of the presence of beta 2m in the cells no association of the transfected class I chain with beta 2m was observed. However, stimulation with IFN-gamma induced assembly and subsequent surface expression. These findings show that the assembly of class I heavy chains with beta 2m is not a spontaneous event but appears to be regulated by cellular mechanisms the nature of which is still unknown.

MeSH Terms
Animals Cell Membrane/immunology,metabolism Gene Expression Regulation/drug effects Genes, MHC Class I/drug effects H-2 Antigens/genetics Interferon-gamma/pharmacology Tumor Cells, Cultured/drug effects,immunology,metabolism beta 2-Microglobulin/genetics,metabolism
Chemicals
H-2 Antigens beta 2-Microglobulin Interferon-gamma
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klar D
Institute of Immunology and Genetics, German Cancer Research Center, Heidelberg.
Hämmerling G J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-02-00
Pages
475-81
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400829
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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