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PMID: 6096831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of the murine H-2Kk gene. Comparison of three H-2K locus alleles.

Nucleic acids research ·Vol. 12 ·No. 24 ·1984-12-21 ·Pages 9473-87

Arnold B, Burgert HG, Archibald AL, Kvist S

Abstract

We have determined the DNA sequence of the H-2Kk gene of the mouse major histocompatibility complex (MHC). Comparison on the nucleotide and protein level of three H-2K alleles (Kk, Kb and Kd) reveals a high degree of homology, in particular between the Kb and Kk alleles. Differences between the two latter antigens are almost exclusively confined to the alpha 1 and alpha 2 domains. At nine positions in the extracellular part of the molecules we have found allele-specific amino acids. Interestingly, 78% of these residues are either polar or carry hydroxyl-groups. This makes it likely that they are exposed on the surface of the molecules and might then be part of antigenic determinants. We have also identified potentially allele-specific nucleotide sequences of the K genes which might be used as specific DNA probes.

MeSH Terms
Alleles Amino Acid Sequence Animals Bacteriophage lambda/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes H-2 Antigens/genetics Mice/genetics
Chemicals
H-2 Antigens DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Arnold B
Burgert H G
Archibald A L
Kvist S
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.24.9473
Published
1984-12-21
Pages
9473-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320474
Subset
IM
Databases
GENBANK
X01652
External Links
PubMed source DOI: 10.1093/nar/12.24.9473 PMC Full Text View journal details
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