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PMID: 1851887 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs.

Journal of virology ·Vol. 65 ·No. 6 ·1991-06-00 ·Pages 3388-94

Cosset FL, Legras C, Thomas JL, Molina RM, Chebloune Y, Faure C, Nigon VM, Verdier G

Abstract

Production and expression of double-expression vectors which transduce both Neo(r) and lacZ genes and are based on the structure of avian leukosis virus were enhanced by using cis-acting sequences (long terminal repeats and noncoding sequences) from Rous-associated virus-1 and Rous-associated virus-2 rather than those of avian erythroblastosis virus previously used in our constructs. Polyclonal producer cells obtained after transfection of these vectors into the Isolde packaging cell line gave rise to titers as high as 3 x 10(5) lacZ CFU/ml, whereas it was possible to isolate clones of producer cells giving rise to titers of more than 10(6) resistance focus-forming units per ml.

MeSH Terms
Animals Avian Leukosis Virus/genetics Cell Line Gene Expression Lac Operon Plasmids RNA, Viral/analysis Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid
Chemicals
RNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cosset F L
Institut National de la Recherche Agronomique, Université Claude Bernard Lyon-I, Villeurbanne, France.
Legras C
Thomas J L
Molina R M
Chebloune Y
Faure C
Nigon V M
Verdier G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.6.3388-3394.1991
Published
1991-06-00
Pages
3388-94
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241003
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.6.3388-3394.1991 PMC Full Text View journal details
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