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PMID: 3029769 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A promoterless retroviral vector indicates that there are sequences in U3 required for 3' RNA processing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 5 ·1987-03-00 ·Pages 1197-201

Dougherty JP, Temin HM

Abstract

We constructed a retrovirus vector in which all viral transcriptional signals are deleted during provirus formation. This vector would be safer and more useful in gene-transfer experiments. Construction of this vector involved the deletion of all of the U3 sequence except for 10 base pairs, required for integration, from the right-side long terminal repeat. We found that this deletion resulted in an inability to propagate virus efficiently. When we inserted sequences containing all of the signals for polyadenylylation of simian virus 40 late mRNA at the end of our vector, we were able to propagate virus efficiently. This result indicates that there are sequences in U3 upstream from AAUAAA that are essential for 3' processing of viral RNA.

MeSH Terms
Animals Base Sequence Cell Line Dogs Endonucleases Genes, Viral Genetic Vectors Promoter Regions, Genetic Retroviridae/genetics Simian virus 40/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dougherty J P
Temin H M
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.5.1197
Published
1987-03-00
Pages
1197-201
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304393
Subset
IM
Grants
NCI NIH HHS · CA-07175 · United States
NCI NIH HHS · CA-09075 · United States
NCI NIH HHS · CA-22443 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.5.1197 PMC Full Text View journal details
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