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PMID: 3502707 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region.

Journal of virology ·Vol. 61 ·No. 5 ·1987-05-00 ·Pages 1639-46

Bender MA, Palmer TD, Gelinas RE, Miller AD

Abstract

Replication-competent retroviruses can be modified to carry nonviral genes. Such gene transfer vectors help define regions of the retroviral genome that are required in cis for retroviral replication. Moloney murine leukemia virus has been used extensively in vector construction, and all of the internal protein-encoding regions can be removed and replaced with other genes while still allowing production of virions containing and transmitting the altered retroviral genome. However, inclusion of a portion of the gag region from Moloney murine leukemia virus markedly increases the titer of virus derived from these vectors. We determined that this effect was due to more efficient packaging of the vector RNA into particles and did not depend on protein synthesis from the gag region. We conclude that the retrovirus packaging signal extends into the gag region. We have found that retroviral vectors containing the complete packaging signal allow more efficient gene transfer into a variety of cell types. In addition, these results may help explain why many oncogenic retroviruses have retained gag sequences and often express transforming proteins that are gag-onc hybrids.

MeSH Terms
Gene Products, gag Genetic Complementation Test Genetic Vectors Moloney murine leukemia virus/genetics,growth & development Morphogenesis RNA Splicing RNA, Viral/genetics Retroviridae Proteins/physiology Virus Replication
Chemicals
Gene Products, gag RNA, Viral Retroviridae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bender M A
Palmer T D
Gelinas R E
Miller A D
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30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.5.1639-1646.1987
Published
1987-05-00
Pages
1639-46
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254146
Subset
IM
Grants
NCI NIH HHS · CA41455 · United States
NHLBI NIH HHS · HL36444 · United States
NHLBI NIH HHS · HL37073 · United States
Databases
GENBANK
J02255, J02256, J02257
External Links
PubMed source DOI: 10.1128/JVI.61.5.1639-1646.1987 PMC Full Text View journal details
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