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PMID: 1849653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of PC3, a putatively secreted protein whose mRNA is induced by nerve growth factor and depolarization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 8 ·1991-04-15 ·Pages 3353-7

Bradbury A, Possenti R, Shooter EM, Tirone F

Abstract

PC3 is an immediate early gene induced by nerve growth factor in PC12 cells, a cell line derived from a tumor of the adrenal medulla that undergoes neuronal differentiation in the presence of nerve growth factor. This induction is independent of new protein synthesis as it can occur in the presence of cycloheximide. PC3 is also induced with similar kinetics, but at lower levels, by membrane depolarization (both in vivo and in vitro) and epidermal growth factor. It is induced at much lower levels by fibroblast growth factor and interleukin 6. In vivo it is found expressed in tissues, such as brain at embryonic day 13.5, placenta, amnion, and spleen, which are proliferating and/or differentiating. The deduced protein sequence from the cDNA indicates the presence of a signal peptide, suggesting that PC3 is secreted.

MeSH Terms
Adrenal Gland Neoplasms/genetics Amino Acid Sequence Animals Base Sequence Bucladesine/pharmacology Cloning, Molecular DNA/genetics Epidermal Growth Factor/pharmacology Fibroblast Growth Factors/pharmacology Gene Expression/drug effects Immediate-Early Proteins/genetics,metabolism Interleukin-6/pharmacology Membrane Potentials/drug effects Molecular Sequence Data Neoplasm Proteins/genetics,metabolism Nerve Growth Factors/pharmacology Nerve Tissue Proteins/genetics,metabolism Pentylenetetrazole/pharmacology Pheochromocytoma/genetics Potassium Chloride/pharmacology RNA, Messenger/genetics Rats Restriction Mapping Tetradecanoylphorbol Acetate/pharmacology Tissue Distribution Tumor Suppressor Proteins
Chemicals
BTG2 protein, rat Immediate-Early Proteins Interleukin-6 Neoplasm Proteins Nerve Growth Factors Nerve Tissue Proteins RNA, Messenger Tumor Suppressor Proteins Fibroblast Growth Factors Epidermal Growth Factor Bucladesine Potassium Chloride DNA Tetradecanoylphorbol Acetate Pentylenetetrazole
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bradbury A
Istituto di Neurobiologia, Consiglio Nazionale delle Ricerche, Rome, Italy.
Possenti R
Shooter E M
Tirone F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.8.3353
Published
1991-04-15
Pages
3353-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51445
Subset
IM
Grants
NINDS NIH HHS · NS 04270 · United States
Databases
GENBANK
M60361, M60362, M60363, M60364, M60365, M60366, M60367, M60368, M60369, M60921
External Links
PubMed source DOI: 10.1073/pnas.88.8.3353 PMC Full Text View journal details
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