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PMID: 3422491 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nerve growth factor induces the genes for two proteins related to a family of calcium-binding proteins in PC12 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 4 ·1988-02-00 ·Pages 1277-81

Masiakowski P, Shooter EM

Abstract

Differential hybridization of a cDNA library from rat pheochromocytoma PC12 cells with cDNA probes from naive PC12 cells and from PC12 cells exposed to nerve growth factor for 7 days identified cDNA sequences of two genes induced by NGF. The mRNA species detected by these cDNA sequences, designated 42A and 42C, reached maximal levels after 24 hr of treatment with NGF and were still significantly higher than control levels after 7 days. Epidermal growth factor transiently induced both mRNAs but at much lower levels. The mRNAs code for 95- (42C) and 101- (42A) amino acid residue peptides whose sequences are homologous to those of a family of calcium-binding proteins including the S-100 protein. The conservation of primary and secondary structure between 42A, 42C, and the other proteins suggests a possible role for them in the regulation of cell growth and differentiation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Calcium-Binding Proteins/biosynthesis,genetics DNA/genetics Gene Expression Regulation/drug effects Genes Molecular Sequence Data Multigene Family Neoplasm Proteins/biosynthesis,genetics Nerve Growth Factors/pharmacology Pheochromocytoma/pathology RNA, Messenger/biosynthesis Rats Sequence Homology, Nucleic Acid Tumor Cells, Cultured/drug effects,metabolism
Chemicals
Calcium-Binding Proteins Neoplasm Proteins Nerve Growth Factors RNA, Messenger DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Masiakowski P
Department of Neurobiology, Stanford University School of Medicine, CA 94305.
Shooter E M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.4.1277
Published
1988-02-00
Pages
1277-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279750
Subset
IM
Grants
NINDS NIH HHS · NS 04270 · United States
Databases
GENBANK
J03627, J03628
External Links
PubMed source DOI: 10.1073/pnas.85.4.1277 PMC Full Text View journal details
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