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PMID: 2467301 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Early gene regulation by nerve growth factor in PC12 cells: induction of an interferon-related gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 6 ·1989-03-00 ·Pages 2088-92

Tirone F, Shooter EM

Abstract

Nerve growth factor (NGF) induces the chromaffin cell line PC12 to differentiate into cells with many of the properties of sympathetic neurons. We investigated the early differentiative phase and identified a gene, PC4, rapidly and transiently induced by NGF in PC12 cells. PC4 cDNA is homologous to the partial sequence of a putative mouse beta-interferon and encodes a protein related to a lymphokine, the rat gamma-interferon protein. Nonetheless, PC4 appears devoid of antiviral activity. PC4 is expressed in proliferating and differentiating tissues, such as amnion, placenta, and brain at embryonic day 13.5. The relationship of PC4 to interferons and lymphokines suggests that it could play a role in regulating gene activity in the pathways induced by NGF.

MeSH Terms
Adrenal Gland Neoplasms Amino Acid Sequence Animals Base Sequence Bucladesine/pharmacology DNA/genetics,isolation & purification Gene Expression Regulation Immediate-Early Proteins/genetics Interferon Type I/genetics Interferon-gamma/genetics Interferons/genetics Membrane Proteins Mice Molecular Sequence Data Nerve Growth Factors/pharmacology Nerve Tissue Proteins/genetics Nucleic Acid Hybridization Pheochromocytoma Plasmids RNA, Messenger/biosynthesis Rats Sequence Homology, Nucleic Acid Tissue Distribution Transfection Tumor Cells, Cultured
Chemicals
Ifrd1 protein, mouse Ifrd1 protein, rat Immediate-Early Proteins Interferon Type I Membrane Proteins Nerve Growth Factors Nerve Tissue Proteins RNA, Messenger Bucladesine Interferon-gamma DNA Interferons
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tirone F
Department of Neurobiology, Stanford University School of Medicine, CA 94305.
Shooter E M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.6.2088
Published
1989-03-00
Pages
2088-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286853
Subset
IM
Grants
NINDS NIH HHS · NS04270 · United States
Databases
GENBANK
J04511
External Links
PubMed source DOI: 10.1073/pnas.86.6.2088 PMC Full Text View journal details
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