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PMID: 2412230 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of endothelial cell growth factor with heparin: characterization by receptor and antibody recognition.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 18 ·1985-09-00 ·Pages 6138-42

Schreiber AB, Kenney J, Kowalski WJ, Friesel R, Mehlman T, Maciag T

Abstract

Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occupancy by the ligand. Heparin interacts structurally with ECGF [Maciag, T., Mehlman, T., Friesel, R. & Schreiber, A. B. (1984) Science 225, 932-935], potentiates the mitogenic activity of the polypeptide, restores the biological activity to inactivate ECGF, enhances the affinity of the ligand to cell surface receptors, and modifies antibody recognition of ECGF. These data suggest that the association between heparin and ECGF induces a conformational change in the polypeptide that increases or stabilizes the biological activity of the mitogen.

MeSH Terms
Cell Division/drug effects Cells, Cultured Endothelial Growth Factors Endothelium/metabolism Epitopes Fibroblasts/metabolism Growth Substances/immunology,metabolism Heparin/pharmacology Humans Mitosis Protein Binding/drug effects Protein Conformation/drug effects Receptors, Mitogen/metabolism Receptors, Vascular Endothelial Growth Factor
Chemicals
Endothelial Growth Factors Epitopes Growth Substances Receptors, Mitogen Heparin Receptors, Vascular Endothelial Growth Factor
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schreiber A B
Kenney J
Kowalski W J
Friesel R
Mehlman T
Maciag T
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.18.6138
Published
1985-09-00
Pages
6138-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391007
Subset
IM
Grants
NIA NIH HHS · AG 04807 · United States
NHLBI NIH HHS · HL 310765 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.18.6138 PMC Full Text View journal details
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