How to make a hybridoma.
The use of monoclonal antibodies produced by the hybridoma technique has had a profound impact on the biological and medical sciences. While the general technique for the production of monoclonal antibodies is available from the numerous papers which report their use, there is a need for a source which describes the procedure and its pitfalls in detail so that an investigator who decides to make hybridomas can do so quickly and easily. This guide provides such a source.
10 references, click to expand
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Continuous cultures of fused cells secreting antibody of predefined specificity.Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
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The isolation of heterokaryons and hybrids by a selective system using irreversible biochemical inhibitors.Exp Cell Res. 1978 Mar 15;112(2):395-407 PMID: 631224
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Monoclonal xenogeneic antibodies to mouse leukocyte antigens: identification of macrophage-specific and other differentiation antigens.Curr Top Microbiol Immunol. 1978;81:45-50 PMID: 688760
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A better cell line for making hybridomas secreting specific antibodies.Nature. 1978 Nov 16;276(5685):269-70 PMID: 714156
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Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.Nature. 1979 Jan 11;277(5692):131-3 PMID: 310519
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Monoclonal antibody against an Ir gene product?J Exp Med. 1980 Oct 1;152(4):1085-101 PMID: 6158546
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Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes.Nature. 1980 Oct 2;287(5781):443-5 PMID: 6253801
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Production of human hybridomas secreting antibodies to measles virus.Nature. 1980 Dec 4;288(5790):488-9 PMID: 6969366
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Antibody production by hybridomas.J Immunol Methods. 1980;39(4):285-308 PMID: 7007515
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SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.Science. 1964 Aug 14;145(3633):709-10 PMID: 14168277
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