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PMID: 1845898 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Determinants of retrovirus gene expression in embryonal carcinoma cells.

Journal of virology ·Vol. 65 ·No. 1 ·1991-01-00 ·Pages 382-8

Akgün E, Ziegler M, Grez M

Abstract

The expression of Moloney murine leukemia virus is restricted in embryonal carcinoma (EC) cells. To characterize specific mutations necessary for expression of retroviruses in EC cells, we analyzed the expression of retrovirus mutants and recombinants thereof in EC cell lines F9 and PCC4. DNA sequence comparison and functional studies allowed us to define three point mutations in the enhancer region of the viral mutants at positions -345, -326, and -166 and two point mutations within the 5'-untranslated region of the viral genome at positions +164 and +165 that were essential for retrovirus expression in EC cells. DNA fragments derived from either the wild type or mutant viruses were used to search for sequence-specific DNA-binding factors in nuclear extracts from undifferentiated PCC4 cells. A cellular factor was found to bind strongly to sequences within the enhancer region (-354 to -306) of wild-type viruses but only weakly to sequences derived from mutant viruses. This factor was named ECF-I (for EC cell factor I). Retroviral expression in EC cells correlates with decreased binding affinity for ECF-I.

MeSH Terms
Animals Base Sequence Cell Line Cell Nucleus/metabolism DNA, Viral/genetics Gene Expression Mammary Tumor Virus, Mouse/genetics Mice Molecular Sequence Data Moloney murine leukemia virus/genetics Moloney murine sarcoma virus/genetics Repetitive Sequences, Nucleic Acid Restriction Mapping Retroviridae/genetics Teratoma Transfection
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Akgün E
Abteilung für Zellbiologie, Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Hamburg, Federal Republic of Germany.
Ziegler M
Grez M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.1.382-388.1991
Published
1991-01-00
Pages
382-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240528
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.1.382-388.1991 PMC Full Text View journal details
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