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PMID: 3172339 Published · ppublish English Journal Article

Negative regulation of retrovirus expression in embryonal carcinoma cells mediated by an intragenic domain.

Journal of virology ·Vol. 62 ·No. 11 ·1988-11-00 ·Pages 4086-95

Loh TP, Sievert LL, Scott RW

Abstract

An intragenic region spanning the tRNA primer binding site of a Moloney murine leukemia virus recombinant retrovirus was found to restrict expression specifically in embryonal carcinoma (EC) cells. When the inhibitory domain was present, the levels of steady-state RNA synthesized from integrated recombinant templates in stable cotransformation assays were reduced 20-fold in EC cells but not in C2 myoblast cells. Transient-cotransfection assays showed that repression of a template containing the EC-specific inhibitory component was relieved by an excess of specific competitor DNA. In addition, repression mediated by the inhibitory component was orientation independent. This evidence demonstrates the presence of a saturable, trans-acting negative regulatory factor(s) in EC cells and suggests that the interaction of the factor(s) with the intragenic inhibitory component occurs at the DNA level.

MeSH Terms
Animals Base Sequence Binding, Competitive DNA, Viral/genetics Embryonal Carcinoma Stem Cells Enhancer Elements, Genetic Gene Expression Regulation Moloney murine leukemia virus/genetics Neoplastic Stem Cells/microbiology Proviruses/genetics RNA Caps RNA, Viral/biosynthesis Recombination, Genetic Retroviridae/genetics Transfection
Chemicals
DNA, Viral RNA Caps RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Loh T P
E. I. du Pont de Nemours and Company, Inc., Central Research and Development Department, Wilmington, Delaware 19898.
Sievert L L
Scott R W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.11.4086-4095.1988
Published
1988-11-00
Pages
4086-95
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253839
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.62.11.4086-4095.1988 PMC Full Text View journal details
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