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PMID: 1840521 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Anatomy of the parp gene promoter of Trypanosoma brucei.

The EMBO journal ·Vol. 10 ·No. 11 ·1991-11-00 ·Pages 3379-86

Sherman DR, Janz L, Hug M, Clayton C

Abstract

While growing in the tsetse fly, Trypanosoma brucei expresses a major surface glycoprotein, the procyclic acidic repetitive protein (PARP). The parp genes are transcribed by an alpha-amanitin-resistant RNA polymerase. We have determined the sequence requirements for parp promoter activity. Studies of RNA produced from input DNA in transiently transfected trypanosomes indicate that the RNA is correctly processed by trans-splicing and polyadenylation. Deletion analyses show that 330 bp are sufficient for full promoter and splicing activity and that the promoter structure is complex, involving at least three elements whose mutual spacing is important. Mutagenesis pin-pointed two sequences vital for promoter activity; neither bears any resemblance to known prokaryotic or eukaryotic promoter elements.

Related Genes
parp
MeSH Terms
Animals Base Sequence Blotting, Northern Chloramphenicol O-Acetyltransferase/genetics Chromosome Deletion Clone Cells Membrane Glycoproteins Molecular Sequence Data Oligonucleotides/genetics Promoter Regions, Genetic Protozoan Proteins RNA Splicing RNA, Messenger/analysis Transcription, Genetic Trypanosoma brucei brucei/genetics Variant Surface Glycoproteins, Trypanosoma/genetics
Chemicals
Membrane Glycoproteins Oligonucleotides Protozoan Proteins RNA, Messenger Variant Surface Glycoproteins, Trypanosoma procyclic acidic repetitive protein, Trypanosoma Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sherman D R
Rockefeller University, New York, NY 10021.
Janz L
Hug M
Clayton C
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49 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-11-00
Pages
3379-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453066
Subset
IM
Grants
NIAID NIH HHS · AI22229 · United States
Databases
GENBANK
S58426, S59882, S60066, S60920, X56822, X58357, X58358, X60659, X60660, X60661
External Links
PubMed source PMC Full Text View journal details
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