The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system.
The DNA sequences required for faithful initiation of ribosomal RNA transcription were determined. BAL-31 digestion was used to modify the rDNA template by introducing deletions from its 3'- and 5'-ends. The resulting mutant DNAs were tested for template activity individually or in competition with wild type utilizing an in vitro transcription system from Acanthamoeba castellanii. The results identify the sequence extending from -31 to +8 to be absolutely required for transcription. In addition; when the region between -47 and -32 is left intact, transcription is augmented.
20 references, click to expand
-
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
-
Faithful initiation of ribosomal RNA transcription from cloned DNA by purified RNA polymerase I.Biochemistry. 1984 Aug 28;23(18):4167-72 PMID: 6091740
-
Regulatory sequences involved in the promotion and termination of RNA transcription.Annu Rev Genet. 1979;13:319-53 PMID: 94251
-
Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
-
Organization and expression of eucaryotic split genes coding for proteins.Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
-
Ribosomal ribonucleic acid repeat unit of Acanthamoeba castellanii: cloning and restriction endonuclease map.Biochemistry. 1981 Jun 23;20(13):3822-7 PMID: 6268147
-
Ribosomal RNA transcription in vitro is species specific.Nature. 1982 Mar 11;296(5853):173-4 PMID: 7063022
-
Transcriptional control signals of a eukaryotic protein-coding gene.Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
-
Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.Proc Natl Acad Sci U S A. 1982 May;79(10):3092-6 PMID: 6954460
-
Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.Cell. 1982 Oct;30(3):835-42 PMID: 7139716
-
Nucleotide sequence requirements for specific initiation of transcription by RNA polymerase I.Proc Natl Acad Sci U S A. 1982 Nov;79(22):6908-11 PMID: 6294665
-
Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.Proc Natl Acad Sci U S A. 1982 Dec;79(23):7132-6 PMID: 6961401
-
Transcription by RNA polymerase III.Curr Top Dev Biol. 1983;18:59-88 PMID: 6340979
-
Regulation of human ribosomal RNA transcription.Proc Natl Acad Sci U S A. 1983 Jun;80(12):3558-62 PMID: 6304717
-
A component of Drosophila RNA polymerase I promoter lies within the rRNA transcription unit.Nature. 1983 Jul 14-20;304(5922):179-81 PMID: 6306476
-
Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.Cell. 1983 Oct;34(3):989-96 PMID: 6627394
-
Determination of the promoter region of mouse ribosomal RNA gene by an in vitro transcription system.Proc Natl Acad Sci U S A. 1984 Jan;81(2):299-303 PMID: 6320178
-
The origin of the rRNA precursor from Xenopus borealis, analysed in vivo and in vitro.Nucleic Acids Res. 1983 Dec 10;11(23):8167-81 PMID: 6324076
-
In vitro mutagenesis and transcriptional analysis of a mouse ribosomal promoter element.Proc Natl Acad Sci U S A. 1984 Apr;81(7):2137-41 PMID: 6326106
-
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.Gene. 1979 May;6(1):23-8 PMID: 383576
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
