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PMID: 6324076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The origin of the rRNA precursor from Xenopus borealis, analysed in vivo and in vitro.

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8167-81

McStay B, Bird A

Abstract

We have determined the origin of the major transcript of Xenopus borealis rDNA by the use of an SI nuclease protection assay. The DNA surrounding the origin of this transcript was sequenced, and the region upstream of the origin was shown to have strong sequence homology with that region from X.laevis rDNA. We have also demonstrated faithful transcription from this origin using cloned X.borealis rDNA in an extract derived from X. laevis culture cells. This in vitro transcription was insensitive to 100 micrograms/ml alpha-amanatin, suggesting that it was mediated by RNA polymerase 1.

MeSH Terms
Animals Base Sequence Cell Line Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Endonucleases Female Oocytes/metabolism Plasmids RNA, Ribosomal/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic Xenopus/genetics
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McStay B
Bird A
References (33)
33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.23.8167
Published
1983-12-10
Pages
8167-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326573
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.23.8167 PMC Full Text View journal details
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