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PMID: 1698609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters.

The EMBO journal ·Vol. 9 ·No. 10 ·1990-10-00 ·Pages 3145-51

Pays E, Coquelet H, Tebabi P, Pays A, Jefferies D, Steinert M, Koenig E, Williams RO, Roditi I

Abstract

The variant surface glycoprotein (VSG) and procyclin are the major surface proteins of the bloodstream and procyclic stages, respectively, of Trypanosoma brucei. The promoter regions of the VSG and procyclin gene transcription units could be mapped thanks to the specific enrichment of initial transcripts that occurs following UV irradiation. Whereas the VSG gene is 45 kb distant from its promoter, procyclin genes are located immediately downstream. We show, by run-on assays on isolated nuclei and by cDNA analysis, that transcription occurs from both promoters in bloodstream as well as in procyclic forms. It is inferred that the control of the stage-specific expression of VSG and procyclin genes is not effected at the level of transcription initiation, but most probably by interfering with the elongation and stability of the specific transcripts.

MeSH Terms
Animals Base Sequence DNA/genetics,isolation & purification Genes Membrane Glycoproteins Molecular Sequence Data Promoter Regions, Genetic Protozoan Proteins RNA/genetics,isolation & purification Restriction Mapping Transcription, Genetic Trypanosoma brucei brucei/genetics,immunology Variant Surface Glycoproteins, Trypanosoma/genetics
Chemicals
Membrane Glycoproteins Protozoan Proteins Variant Surface Glycoproteins, Trypanosoma procyclic acidic repetitive protein, Trypanosoma RNA DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Pays E
Department of Molecular Biology, University of Brussels, Belgium.
Coquelet H
Tebabi P
Pays A
Jefferies D
Steinert M
Koenig E
Williams R O
Roditi I
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-10-00
Pages
3145-51
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552043
Subset
IM
Databases
GENBANK
M27962, M27963, M27964, M58572, M58573, M58574
External Links
PubMed source PMC Full Text View journal details
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