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PMID: 3366120 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.

The EMBO journal ·Vol. 7 ·No. 2 ·1988-02-00 ·Pages 455-63

Tschudi C, Ullu E

Abstract

In African trypanosomes, calmodulin is encoded by a small family of tandemly repeated genes consisting of three to four units. We show that all the members of the calmodulin cluster of Trypanosoma brucei gambiense are expressed. In addition to mature mRNAs, steady-state RNA contains a small percentage of polygene transcripts which comprise at least two and probably all calmodulin genes. The 5' ends of a portion of these molecules appear to be indistinguishable from those of mature calmodulin mRNAs. Polygene transcripts are not polyadenylated and have discrete ends which map in the intergenic regions downstream from the polyadenylation sites. Using biotinylated hybridization probes and selection of the hybrids on streptavidin-agarose, we further show that calmodulin polygene transcripts are the most abundant RNA species detected in pulse-labelled RNA of cultured procyclic trypanosomes. Our data strongly imply that polygene transcripts are authentic precursors to mature calmodulin mRNAs.

MeSH Terms
Animals Calmodulin/genetics Cloning, Molecular DNA, Ribosomal/genetics Genes Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Transcription, Genetic Trypanosoma brucei brucei/genetics Trypanosoma brucei gambiense/genetics
Chemicals
Calmodulin DNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tschudi C
Yale MacArthur Center for Molecular Parasitology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
Ullu E
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32 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-02-00
Pages
455-63
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454342
Subset
IM
Grants
NIAID NIH HHS · AI08614 · United States
External Links
PubMed source PMC Full Text View journal details
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