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PMID: 18332108 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription regulation of the rRNA gene by a multifunctional nucleolar protein, B23/nucleophosmin, through its histone chaperone activity.

Molecular and cellular biology ·Vol. 28 ·No. 10 ·2008-05-00 ·Pages 3114-26

Murano K, Okuwaki M, Hisaoka M, Nagata K

Abstract

It is well established that the transcription rate of the rRNA gene is closely associated with profound alterations in the cell growth rate. Regulation of rRNA gene transcription is likely to be dependent on the dynamic conversion of the chromatin structure. Previously, we identified B23/nucleophosmin, a multifunctional nucleolar phosphoprotein, as a component of template activating factor III that remodels the chromatin-like structure of the adenovirus genome complexed with viral basic proteins. It has also been shown that B23 has histone chaperone activity. Here, we examined the effect of B23 on rRNA gene transcription. B23 was found to be associated with the rRNA gene chromatin. Small-interfering-RNA-mediated down-regulation of the B23 expression level resulted in reduction of the transcription rate of the rRNA gene. We constructed a B23 mutant termed B23DeltaC, which lacks the domain essential for the histone chaperone activity and inhibited the histone binding activity of B23 in a dominant-negative manner. Expression of B23DeltaC decreased rRNA gene transcription and the rate of cell proliferation. These results suggest that B23 is involved in the transcription regulation of the rRNA gene as a nucleolar histone chaperone.

MeSH Terms
Animals Base Sequence Cell Line Cell Proliferation Chromatin Assembly and Disassembly DNA, Complementary/genetics Gene Deletion Genes, p53 HeLa Cells Histones/metabolism Humans Mice Molecular Chaperones/metabolism Nuclear Proteins/antagonists & inhibitors,genetics,metabolism Nucleophosmin RNA, Ribosomal/genetics RNA, Small Interfering/genetics Transcription, Genetic
Chemicals
DNA, Complementary Histones Molecular Chaperones NPM1 protein, human Npm1 protein, mouse Nuclear Proteins RNA, Ribosomal RNA, Small Interfering Nucleophosmin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Murano Kensaku
Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Okuwaki Mitsuru
Hisaoka Miharu
Nagata Kyosuke
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.02078-07
Published
2008-05-00
Epub
2008-00-10
Pages
3114-26
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2423177
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.02078-07 PMC Full Text View journal details
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