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PMID: 17466624 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Transcription elongation by RNA polymerase I is linked to efficient rRNA processing and ribosome assembly.

Molecular cell ·Vol. 26 ·No. 2 ·2007-04-27 ·Pages 217-29

Schneider DA, Michel A, Sikes ML, Vu L, Dodd JA, Salgia S, Osheim YN, Beyer AL, Nomura M

Abstract

The synthesis of ribosomes in eukaryotic cells is a complex process involving many nonribosomal protein factors and snoRNAs. In general, the processes of rRNA transcription and ribosome assembly are treated as temporally or spatially distinct. Here, we describe the identification of a point mutation in the second largest subunit of RNA polymerase I near the active center of the enzyme that results in an elongation-defective enzyme in the yeast Saccharomyces cerevisiae. In vivo, this mutant shows significant defects in rRNA processing and ribosome assembly. Taken together, these data suggest that transcription of rRNA by RNA polymerase I is linked to rRNA processing and maturation. Thus, RNA polymerase I, elongation factors, and rRNA sequence elements appear to function together to optimize transcription elongation, coordinating cotranscriptional interactions of many factors/snoRNAs with pre-rRNA for correct rRNA processing and ribosome assembly.

MeSH Terms
Genes, Fungal Point Mutation Protein Subunits RNA Polymerase I/chemistry,genetics,metabolism RNA Processing, Post-Transcriptional RNA, Fungal/metabolism RNA, Ribosomal/metabolism Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic
Chemicals
Protein Subunits RNA, Fungal RNA, Ribosomal RNA Polymerase I
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Schneider David A
Department of Biological Chemistry, University of California, Irvine, 240-D Medical Sciences I, Irvine, CA 92697-1700, USA.
Michel Antje
Sikes Martha L
Vu Loan
Dodd Jonathan A
Salgia Shilpa
Osheim Yvonne N
Beyer Ann L
Nomura Masayasu
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-2765
DOI
10.1016/j.molcel.2007.04.007
Published
2007-04-27
Pages
217-29
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC1927085
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035949-24 · United States
NIGMS NIH HHS · GM-63952 · United States
NIGMS NIH HHS · GM-35949 · United States
NIGMS NIH HHS · R01 GM063952 · United States
NIGMS NIH HHS · R01 GM035949 · United States
External Links
PubMed source DOI: 10.1016/j.molcel.2007.04.007 PMC Full Text View journal details
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