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PMID: 18327244 Published · ppublish English Guideline Journal Article Research Support, N.I.H., Extramural

Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE).

Nature biotechnology ·Vol. 26 ·No. 3 ·2008-03-00 ·Pages 305-12

Deutsch EW, Ball CA, Berman JJ, Bova GS, Brazma A, Bumgarner RE, Campbell D, Causton HC, Christiansen JH, Daian F, Dauga D, Davidson DR, Gimenez G, Goo YA, Grimmond S, Henrich T, Herrmann BG, Johnson MH, Korb M, Mills JC, Oudes AJ, Parkinson HE, Pascal LE, Pollet N, Quackenbush J, Ramialison M, Ringwald M, Salgado D, Sansone SA, Sherlock G, Stoeckert CJ, Swedlow J, Taylor RC, Walashek L, Warford A, Wilkinson DG, Zhou Y, Zon LI, Liu AY, True LD

Abstract

One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.

MeSH Terms
Computational Biology/methods,standards Gene Expression Profiling/methods,standards Immunohistochemistry/methods,standards In Situ Hybridization/methods,standards
Authors & Affiliations
40 authors, click to expand affiliations / ORCID
Deutsch Eric W
Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98103, USA.
Ball Catherine A
Berman Jules J
Bova G Steven
Brazma Alvis
Bumgarner Roger E
Campbell David
Causton Helen C
Christiansen Jeffrey H
Daian Fabrice
Dauga Delphine
Davidson Duncan R
Gimenez Gregory
Goo Young Ah
Grimmond Sean
Henrich Thorsten
Herrmann Bernhard G
Johnson Michael H
Korb Martin
Mills Jason C
Oudes Asa J
Parkinson Helen E
Pascal Laura E
Pollet Nicolas
Quackenbush John
Ramialison Mirana
Ringwald Martin
Salgado David
Sansone Susanna-Assunta
Sherlock Gavin
Stoeckert Christian J
Swedlow Jason
Taylor Ronald C
Walashek Laura
Warford Anthony
Wilkinson David G
Zhou Yi
Zon Leonard I
Liu Alvin Y
True Lawrence D
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Article Info
Journal
Nature biotechnology
Abbr.
Nat Biotechnol
ISSN
1546-1696
DOI
10.1038/nbt1391
Published
2008-03-00
Pages
305-12
Language
English
Region
United States
NLM ID
9604648
PMCID
PMC4367930
Subset
IM
Grants
NHLBI NIH HHS · R33 HL073712 · United States
NIDDK NIH HHS · DK63481 · United States
NIDDK NIH HHS · DK63630 · United States
NIDDK NIH HHS · DK63328 · United States
NIDDK NIH HHS · U01 DK063483 · United States
NIDDK NIH HHS · DK63483 · United States
NIDDK NIH HHS · U01 DK063481 · United States
NIDDK NIH HHS · DK63400 · United States
NIDDK NIH HHS · R01 DK079798-01A2 · United States
Medical Research Council · MC_U127527203 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/E025080/1 · United Kingdom
NIDDK NIH HHS · R01 DK063400 · United States
NIDDK NIH HHS · U01 DK063328 · United States
NIDDK NIH HHS · R01 DK079798 · United States
Medical Research Council · MC_U117532048 · United Kingdom
NIDDK NIH HHS · U01 DK063630 · United States
External Links
PubMed source DOI: 10.1038/nbt1391 PMC Full Text View journal details
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